濒危棕榈物种 Areca concinna Thwaites 体外繁殖技术的标准化,以促进其保护和利用

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Aparna Veluru, Neema Mohamed, Sandip Shil, Krishna Prakash, K. Kavya, S. Anand, Sudha Raju
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引用次数: 0

摘要

Areca concinna Thwaites(棕榈科)是斯里兰卡西南部特有的濒危植物物种,主要分布在斯里兰卡、印度和东南亚。由于栖息地的丧失,这种棕榈被世界自然保护联盟(IUCN)列为濒危植物物种。克隆繁殖对于保护和维持这一濒危物种至关重要。为了保护这一植物物种,我们制定了 A. concinna Thwaites 的克隆繁殖方案。在添加了辅助剂 2,4-D 和毒莠定的 M72 基础培养基中,对未成熟花序、未成熟胚和成熟胚进行了唤醒发生和随后的体细胞胚胎发生测试。在三种外植体中,只有成熟胚获得了体细胞胚。在添加了 2,4-D 的 M72 基础培养基中,胼胝体的繁殖和体细胞胚的形成优于毒莠定培养基。将外植体从高浓度连续转移到低浓度对维持胼胝体的繁殖和体细胞胚的诱导至关重要。在添加了 1.0 mg L-1 BAP、0.5 mg L-1 NAA 和 0.25 mg L-1 IBA 的 Y3 或 1/2 Y3 基础培养基中生长的体细胞胚表现出芽萌发和小植株发育。该方案可用于快速繁殖 A. concinna Thwaites 棕榈树,从而提高保护这种濒危棕榈树的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Standardization of in vitro multiplication technique for Areca concinna Thwaites, an endangered palm species for its conservation and utilization

Standardization of in vitro multiplication technique for Areca concinna Thwaites, an endangered palm species for its conservation and utilization

Areca concinna Thwaites (Arecaceae) is an endangered plant species endemic to South-Western Sri Lanka and distributed primarily in Sri Lanka, India, and Southeast Asia. This palm was listed under endangered plants species by IUCN (The International Union for Conservation of Nature) due to habitat loss. Clonal propagation is essential for conservation and maintenance of this endangered species. In an effort to conserve this plant species, a protocol was developed for clonal multiplication of A. concinna Thwaites. Immature inflorescence, immature embryos, and mature embryos were tested for callogenesis and subsequent somatic embryogenesis in M72 basal medium supplemented with the auxins 2,4-D and picloram. Of the three explants, somatic embryos were obtained only from mature embryos. Callus multiplication and somatic embryo formation in M72 basal medium supplemented with 2,4-D were found to be better over picloram media. Serial transfer of explants from higher to lower concentrations was essential for sustaining the multiplication of callus as well as for induction of somatic embryos. Somatic embryos grown in Y3 or 1/2 Y3 basal medium supplemented with 1.0 mg L−1 BAP, 0.5 mg L−1 NAA, and 0.25 mg L−1 IBA exhibited shoot initiation and plantlet development. This protocol has its application in rapid multiplication of A. concinna Thwaites palms thereby enhancing the possibility of conservation of the endangered palm.

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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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