Diego Thiers Oliveira Carneiro, Michael Douglas da Silva, Karla Vanessa Pinto Vasconcelos, Romero Dias, Vanessa Costa, Raquel Felipe Vasconcelos, Bárbara Carneiro, Gisele Barreto, Mirna Marques, Hellíada Chaves Vasconcelos, Howard Lopes Ribeiro Júnior, Jonas Nogueira Ferreira Maciel Gusmão, Helson da Silveira, Renata Leitão, Gerly Anne Brito, Karuza Maria Alves Pereira, Delane Viana Gondim, Paula Goes
{"title":"安博藜芦能减轻牙周炎大鼠的骨质流失","authors":"Diego Thiers Oliveira Carneiro, Michael Douglas da Silva, Karla Vanessa Pinto Vasconcelos, Romero Dias, Vanessa Costa, Raquel Felipe Vasconcelos, Bárbara Carneiro, Gisele Barreto, Mirna Marques, Hellíada Chaves Vasconcelos, Howard Lopes Ribeiro Júnior, Jonas Nogueira Ferreira Maciel Gusmão, Helson da Silveira, Renata Leitão, Gerly Anne Brito, Karuza Maria Alves Pereira, Delane Viana Gondim, Paula Goes","doi":"10.30476/dentjods.2023.95767.1891","DOIUrl":null,"url":null,"abstract":"<p><strong>Statement of the problem: </strong>Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. <i>Chenopodium ambrosioides</i> L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis.</p><p><strong>Purpose: </strong>This study aimed to evaluate the bone protective effect of <i>Chenopodium ambrosioides</i> L. (CAL) extract on ligature-induced periodontitis model in rats.</p><p><strong>Materials and method: </strong>For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11<sup>th</sup> day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract.</p><p><strong>Results: </strong><i>Chenopodium ambrosioides</i> L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (<i>p</i>< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.</p><p><strong>Conclusion: </strong>In summary, it was observed that <i>Chenopodium ambrosioides</i> L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent.</p>","PeriodicalId":73702,"journal":{"name":"Journal of dentistry (Shiraz, Iran)","volume":"25 1","pages":"59-67"},"PeriodicalIF":0.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963862/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Chenopodium Ambrosioides</i> Linn Mitigates Bone Loss in Rats with Periodontitis.\",\"authors\":\"Diego Thiers Oliveira Carneiro, Michael Douglas da Silva, Karla Vanessa Pinto Vasconcelos, Romero Dias, Vanessa Costa, Raquel Felipe Vasconcelos, Bárbara Carneiro, Gisele Barreto, Mirna Marques, Hellíada Chaves Vasconcelos, Howard Lopes Ribeiro Júnior, Jonas Nogueira Ferreira Maciel Gusmão, Helson da Silveira, Renata Leitão, Gerly Anne Brito, Karuza Maria Alves Pereira, Delane Viana Gondim, Paula Goes\",\"doi\":\"10.30476/dentjods.2023.95767.1891\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Statement of the problem: </strong>Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. <i>Chenopodium ambrosioides</i> L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis.</p><p><strong>Purpose: </strong>This study aimed to evaluate the bone protective effect of <i>Chenopodium ambrosioides</i> L. (CAL) extract on ligature-induced periodontitis model in rats.</p><p><strong>Materials and method: </strong>For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11<sup>th</sup> day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract.</p><p><strong>Results: </strong><i>Chenopodium ambrosioides</i> L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (<i>p</i>< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.</p><p><strong>Conclusion: </strong>In summary, it was observed that <i>Chenopodium ambrosioides</i> L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent.</p>\",\"PeriodicalId\":73702,\"journal\":{\"name\":\"Journal of dentistry (Shiraz, Iran)\",\"volume\":\"25 1\",\"pages\":\"59-67\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963862/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of dentistry (Shiraz, Iran)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.30476/dentjods.2023.95767.1891\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of dentistry (Shiraz, Iran)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30476/dentjods.2023.95767.1891","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis.
Statement of the problem: Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis.
Purpose: This study aimed to evaluate the bone protective effect of Chenopodium ambrosioides L. (CAL) extract on ligature-induced periodontitis model in rats.
Materials and method: For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11th day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract.
Results: Chenopodium ambrosioides L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (p< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.
Conclusion: In summary, it was observed that Chenopodium ambrosioides L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent.
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