i909c 和 40cC 之间脱细胞和冻干人体真皮的粘弹性能。

IF 1 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2024-01-01
Q P Jia, S L Huang, Y T Tang, W Q Sun
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引用次数: 0

摘要

背景:人类供体皮肤经处理后可制成用于组织修复和再生的无细胞真皮基质(ADM)。目前还没有关于 ADM 在室温和零度以下的粘弹性能的数据:这项工作评估了冻干 ADM 粘弹性能的温度依赖性:对供体皮肤进行去表皮、去细胞和冷冻干燥处理,并使用妥尔糖作为低温保护剂。用差示扫描量热仪(DSC)测量冻干 ADM 的玻璃化转变,用动态机械分析仪(DMA)检测其粘弹性:在低水分范围(1.4 +/- 0.5%)内,冻干 ADM 的玻璃化转变温度(Tg)为 90 摄氏度至 100 摄氏度。冻干 ADM 的储藏模量(G')、损耗模量(G")和阻尼系数 tan delta(G"/G')的变化表明,供体与供体之间的粘弹性有很大差异。不过,所有 8 种供体的 G'、G "和 tan delta 随温度变化的趋势相似。对于每种供体,G'和 G "在-90 摄氏度和 40 摄氏度之间的变化相对较小,G'比 G "至少大一个数量级。在冷冻干燥的 ADM 中观察到两种粘弹性松弛,一种是在-20 摄氏度时,另一种是在-60 摄氏度时:结论:冻干 ADM 在玻璃状碳水化合物基质中受到保护。DMA 观察到两种粘弹性松弛(即 -20 摄氏度时的α过程和 -60 摄氏度时的β过程)。在-90 摄氏度和 40 摄氏度之间,冻干 ADM 的 G' 和 G'' 的总体变化在一个数量级内相对较小。https://doi.org/10.54680/fr24110110612。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Viscoelastic properties of decellularized and freeze-dried human dermis between i909c and 40cC.

Background: Human donor skin is processed to make the acellular dermis matrix (ADM) for tissue repair and regeneration. There is no data on the viscoelastic properties of ADM at room and subzero temperatures.

Objective: The work evaluated the temperature dependence of viscoelastic properties of freeze-dried ADM.

Materials and methods: Donor skin was de-epidermized, de-cellularized and freeze-dried with trehalose as the lyo-protectant. Glass transition of freeze-dried ADM was measured by differential scanning calorimeter (DSC), and viscoelastic properties were examined by dynamic mechanical analyzer (DMA).

Results: At the low moisture range (1.4 +/- 0.5%), the glass transition temperature (Tg) of freeze-dried ADM was 90 degree C to 100 degree C. As the moisture content increased, the Tg decreased steadily. At the high moisture range (10.8 +/- 2.9%), the Tg was 40 degree C to 60 degree C. There were large donor-to-donor variations in viscoelastic properties of freeze-dried ADM as demonstrated by the changes in storage modulus (G'), loss modulus (G") and damping factor tan delta (G"/G'). However, the trends of the temperature dependence for G', G" and tan delta were similar among all 8 donors. For each donor, changes in G' and G" were relatively small between -90 degree C and 40 degree C, and G' was at least one order of magnitude greater than G". Two viscoelastic relaxations were observed in freeze-dried ADM, one at -20 degree C and the other at -60 degree C respectively.

Conclusion: Freeze-dried ADM was protected in the glassy carbohydrate matrix. DMA observed two viscoelastic relaxations (i.e., alpha process at -20 degree C and beta process at -60 degree C). Overall changes in G' and G'' of freeze-dried ADM were relatively small within one order of magnitude between -90 degree C and 40 degree C. https://doi.org/10.54680/fr24110110612.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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