用乳液溶剂蒸发法制备用于化学发光免疫测定的磁性聚苯乙烯纳米粒子

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Chemical & pharmaceutical bulletin Pub Date : 2024-01-01 DOI:10.1248/cpb.c23-00864

Hiroaki Ichimaru, Masashi Kurimoto, Shigetoshi Kikuchi

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引用次数: 0

摘要

在临床诊断中，磁性聚苯乙烯纳米粒子（MPS NPs）通常用于化学发光免疫测定（CLEIA）等。然而，传统的 MPS NPs 制备方法需要长时间加热才能形成聚合物颗粒，效率低下。在本研究中，我们采用乳液溶剂蒸发法制备 MPS NPs，无需加热。我们评估了水相和有机相中的溶剂对磁性粒子含量的影响。用 4% (v/v) MeOH 水溶液并添加硬脂酸（SA）制备的 MPS NPs（4MeSA-MPS NPs）表现出最高的磁性颗粒含量。此外，CLEIA 分析表明，C 反应蛋白的检测限为 80 pg/mL。因此，4MeSA-MPS NPs 在临床诊断中大有可为。

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Magnetic Polystyrene Nanoparticles Prepared by Emulsion Solvent-Evaporation for the Chemiluminescent Immunoassay.

In clinical diagnosis, magnetic polystyrene nanoparticles (MPS NPs) are commonly applied to, e.g., the chemiluminescent immunoassay (CLEIA). However, the conventional preparation method of MPS NPs requires a long duration of heating to form polymer particles, which is inefficient. In this study, we prepared MPS NPs by emulsion solvent-evaporation without heating. We evaluated the effect of the solvent in the water and organic phases on the magnetic particle content. MPS NPs prepared by 4% (v/v) MeOH aqueous solution and adding stearic acid (SA) (4MeSA-MPS NPs) exhibited the highest magnetic particle content. Furthermore, CLEIA analysis indicates that the C-reactive protein detection limit is 80 pg/mL. Thus, 4MeSA-MPS NPs are promising for clinical diagnoses.

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来源期刊

Chemical & pharmaceutical bulletin 医学-化学综合

CiteScore

3.20

自引率

5.90%

发文量

132

审稿时长

1.7 months

期刊介绍： The CPB covers various chemical topics in the pharmaceutical and health sciences fields dealing with biologically active compounds, natural products, and medicines, while BPB deals with a wide range of biological topics in the pharmaceutical and health sciences fields including scientific research from basic to clinical studies. For details of their respective scopes, please refer to the submission topic categories below. Topics: Organic chemistry In silico science Inorganic chemistry Pharmacognosy Health statistics Forensic science Biochemistry Pharmacology Pharmaceutical care and science Medicinal chemistry Analytical chemistry Physical pharmacy Natural product chemistry Toxicology Environmental science Molecular and cellular biology Biopharmacy and pharmacokinetics Pharmaceutical education Chemical biology Physical chemistry Pharmaceutical engineering Epidemiology Hygiene Regulatory science Immunology and microbiology Clinical pharmacy Miscellaneous.