傅立叶变换红外显微镜在人体组织切片中诊断囊性棘球蚴病的适用性。

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2024-03-26 DOI:10.1002/jbio.202300513

A. Brunner, S. H. Unterberger, H. Auer, T. Hautz, S. Schneeberger, R. Stalder, J. Badzoka, C. Kappacher, C. W. Huck, B. Zelger, J. D. Pallua

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引用次数: 0

摘要

囊性棘球蚴病（CE）是一种由绦虫引起的全球性健康问题，由于症状不特异、放射学检查结果不确定，给诊断带来了挑战。诊断有赖于对受影响组织进行组织病理学评估，这需要全面的工具。在这项回顾性病例研究中，傅立叶变换红外显微镜被用于通过人体组织切片的生化变化来检测和识别 CE。研究分析了 11 名确诊 CE 患者的组织样本。对存档的 FFPE 切片进行切割和染色，然后在脱石蜡后使用傅立叶变换红外显微镜检查 CE 阳性的未染色切片。结果显示，无论器官类型如何，该方法都能区分人体组织中的棘球蚴成分。这项研究展示了中红外显微镜作为一种有价值的棘球蚴诊断工具的潜力，有望在面对该疾病的复杂性时提高诊断的精确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Suitability of Fourier transform infrared microscopy for the diagnosis of cystic echinococcosis in human tissue sections

查看原文本刊更多论文

Suitability of Fourier transform infrared microscopy for the diagnosis of cystic echinococcosis in human tissue sections

Cystic echinococcosis (CE) is a global health concern caused by cestodes, posing diagnostic challenges due to nonspecific symptoms and inconclusive radiographic results. Diagnosis relies on histopathological evaluation of affected tissue, demanding comprehensive tools. In this retrospective case study, Fourier transform infrared microscopy was explored for detecting and identifying CE through biochemical changes in human tissue sections. Tissue samples from 11 confirmed CE patients were analyzed. Archived FFPE blocks were cut and stained, and then CE-positive unstained sections were examined using Fourier transform infrared microscopy post-deparaffinization. Results revealed the method's ability to distinguish echinococcus elements from human tissue, irrespective of organ type. This research showcases the potential of mid-infrared microscopy as a valuable diagnostic tool for CE, offering promise in enhancing diagnostic precision in the face of the disease's complexities.

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.