在秀丽隐杆线虫体内通过内源性 RNA 干扰实现整合转基因的组织特异性沉默。

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
RNA Biology Pub Date : 2024-01-01 Epub Date: 2024-03-26 DOI:10.1080/15476286.2024.2332856
Siyu Chen, Weihong Liu, Lei Xiong, Zhiju Tao, Di Zhao
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引用次数: 0

摘要

转基因沉默是在秀丽隐杆线虫(Caenorhabditis elegans)中观察到的一种常见现象,尤其是在种系中。通过分析秀丽隐杆线虫的转录因子谱,我们发现几种转基因报告基因株的表达表现出组织特异性沉默,尤其是在秀丽隐杆线虫的肠道中。值得注意的是,这种沉默在内源性 RNA 干扰(RNAi)缺陷突变体中可以逆转。利用基因敲入菌株进行的进一步研究发现,这些肠道沉默基因确实在体内表达,这表明生物体本身调节着肠道特异性沉默。这种组织特异性沉默似乎是通过内RNAi途径介导的,该途径的主要因子mut-2和mut-16在肠道中明显富集。此外,组蛋白修饰因子(如 met-2)也参与了这种沉默机制。鉴于肠道在生殖过程中与生殖系一起发挥着至关重要的作用,在肠道中观察到的转基因沉默反映了生物体所采用的自我保护机制。总之,我们的研究提出,与其他组织相比,肠道的转基因沉默是由内RNAi途径特异性调控的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tissue-specific silencing of integrated transgenes achieved through endogenous RNA interference in Caenorhabditis elegans.

Transgene silencing is a common phenomenon observed in Caenorhabditis elegans, particularly in the germline, but the precise mechanisms underlying this process remain elusive. Through an analysis of the transcription factors profile of C. elegans, we discovered that the expression of several transgenic reporter lines exhibited tissue-specific silencing, specifically in the intestine of C. elegans. Notably, this silencing could be reversed in mutants defective in endogenous RNA interference (RNAi). Further investigation using knock-in strains revealed that these intestine-silent genes were indeed expressed in vivo, indicating that the organism itself regulates the intestine-specific silencing. This tissue-specific silencing appears to be mediated through the endo-RNAi pathway, with the main factors of this pathway, mut-2 and mut-16, are significantly enriched in the intestine. Additionally, histone modification factors, such as met-2, are involved in this silencing mechanism. Given the crucial role of the intestine in reproduction alongside the germline, the transgene silencing observed in the intestine reflects the self-protective mechanisms employed by the organisms. In summary, our study proposed that compared to other tissues, the transgenic silencing of intestine is specifically regulated by the endo-RNAi pathway.

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来源期刊
RNA Biology
RNA Biology 生物-生化与分子生物学
CiteScore
8.60
自引率
0.00%
发文量
82
审稿时长
1 months
期刊介绍: RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research. RNA Biology brings together a multidisciplinary community of scientists working in the areas of: Transcription and splicing Post-transcriptional regulation of gene expression Non-coding RNAs RNA localization Translation and catalysis by RNA Structural biology Bioinformatics RNA in disease and therapy
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