Chunzhe Lu, Rene H. Wijffels, Vitor A. P. Martins dos Santos, Ruud A. Weusthuis
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引用次数: 0
摘要
中链长度的α,ω-二元醇(mcl-diols)在聚合物生产中发挥着重要作用,传统上依赖于能源密集型的化学工艺。微生物细胞工厂提供了另一种选择,但由于醇和酸等中间产物的毒性,大肠杆菌和酿酒酵母等传统菌株在生产 mcl-二醇时面临挑战。代谢工程和合成生物学使非模式菌株的工程化成为可能,而 P. putida 正成为一个前景广阔的微生物平台。本研究回顾了利用 P. putida 生产二元醇的进展,并提出了一种可持续生产二元醇的四模块方法。尽管取得了进展,但挑战依然存在,本研究讨论了利用普氏拟杆菌作为微生物细胞工厂生产 mcl-二元醇的当前障碍和未来机遇。此外,本研究还强调了将普氏拟杆菌作为二元醇合成的高效底盘的潜力。
Pseudomonas putida as a platform for medium-chain length α,ω-diol production: Opportunities and challenges
Medium-chain-length α,ω-diols (mcl-diols) play an important role in polymer production, traditionally depending on energy-intensive chemical processes. Microbial cell factories offer an alternative, but conventional strains like Escherichia coli and Saccharomyces cerevisiae face challenges in mcl-diol production due to the toxicity of intermediates such as alcohols and acids. Metabolic engineering and synthetic biology enable the engineering of non-model strains for such purposes with P. putida emerging as a promising microbial platform. This study reviews the advancement in diol production using P. putida and proposes a four-module approach for the sustainable production of diols. Despite progress, challenges persist, and this study discusses current obstacles and future opportunities for leveraging P. putida as a microbial cell factory for mcl-diol production. Furthermore, this study highlights the potential of using P. putida as an efficient chassis for diol synthesis.
期刊介绍:
Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes