Lei-Lei Zhao, Jia-Jiao Luo, Jing Cui, Xuan Li, Ruo-Nan Hu, Xin-Yue Xie, Yan-Jing Zhang, Wei Ding, Liang-Ju Ning, Jing-Cong Luo and Ting-Wu Qin*,
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引用次数: 0
摘要
肌腱再生在很大程度上受到氧化剂和炎症微环境的影响。肌腱修复过程中的持续炎症可导致基质降解、肌腱粘连和活性氧(ROS)过度积累,而过量的 ROS 会影响细胞外基质重塑和肌腱整合。在此,我们利用单宁酸(TA)对脱细胞肌腱切片(DTS)进行改性,制造出一种具有抗氧化和抗炎特性的功能性支架(DTS-TA),用于肌腱修复。对支架的特性和细胞相容性进行了体外检测。对支架的抗氧化和抗炎活性进行了体外评估,并使用皮下植入模型对其进行了进一步的体内研究。研究发现,改性后的 DTS 通过氢键和共价键与 TA 结合,支架的亲水性、热稳定性、生物降解性和机械特性都得到了显著改善。随后的研究结果表明,DTS-TA 可提高巨噬细胞 M2/M1 比率和白细胞介素-4(IL-4)的表达,减少白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)的分泌,并清除体内外过多的 ROS,从而有效减轻炎症反应。总之,用 TA 修饰的 DTS 为肌腱再生提供了一种潜在的多功能支架。
Tannic Acid-Modified Decellularized Tendon Scaffold with Antioxidant and Anti-Inflammatory Activities for Tendon Regeneration
Tendon regeneration is greatly influenced by the oxidant and the inflammatory microenvironment. Persistent inflammation during the tendon repair can cause matrix degradation, tendon adhesion, and excessive accumulation of reactive oxygen species (ROS), while excessive ROS affect extracellular matrix remodeling and tendon integration. Herein, we used tannic acid (TA) to modify a decellularized tendon slice (DTS) to fabricate a functional scaffold (DTS-TA) with antioxidant and anti-inflammatory properties for tendon repair. The characterizations and cytocompatibility of the scaffolds were examined in vitro. The antioxidant and anti-inflammatory activities of the scaffold were evaluated in vitro and further studied in vivo using a subcutaneous implantation model. It was found that the modified DTS combined with TA via hydrogen bonds and covalent bonds, and the hydrophilicity, thermal stability, biodegradability, and mechanical characteristics of the scaffold were significantly improved. Afterward, the results demonstrated that DTS-TA could effectively reduce inflammation by increasing the M2/M1 macrophage ratio and interleukin-4 (IL-4) expression, decreasing the secretion of interleukin-6 (IL-6) and interleukin-1β (IL-1β), as well as scavenging excessive ROS in vitro and in vivo. In summary, DTS modified with TA provides a potential versatile scaffold for tendon regeneration.
期刊介绍:
ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.