开发用于选择性捕获癌细胞的 L-苯丙氨酸接枝聚乙烯醇支架

IF 4.1 Q1 CHEMISTRY, ANALYTICAL
Ryoto Itani , Ryo Mitsuyasu , Kang Dong-hee , Kenta Homma , Michiya Matsusaki
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引用次数: 0

摘要

乳腺癌是最常见的癌症,也是女性癌症死亡的主要原因。大多数乳腺癌患者的死亡原因不是原发肿瘤本身,而是转移到了身体的其他器官。在此,我们报告了一种新型聚合物材料的开发情况,这种材料可选择性地捕获乳腺癌细胞。这种捕获癌细胞的聚合物是通过将 L-苯丙氨酸接枝到生物相容性聚乙烯醇(PVA-Phe)上合成的。L-苯丙氨酸对 L 型氨基酸转运体 1(LAT1)具有高亲和力,而 LAT1 在各种癌细胞中都有过表达。我们使用表达 LAT1 的人类激素受体阳性乳腺癌模型细胞 MCF-7,评估了 PVA-Phe 对癌细胞的选择性粘附能力。我们的结果表明,癌细胞上的 LAT1 能识别 L-苯丙氨酸,MCF-7 能选择性地粘附在合成的 PVA-Phe 上。对于有癌症复发风险的患者,我们希望本研究合成的 PVA-Phe 能在植入体内时捕获残留的癌细胞。PVA-Phe与具有吸引癌细胞功能的适当支架一起,可能具有完全防止癌症复发的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of L-phenylalanine-grafted poly (vinyl alcohol) scaffold for selective capturing of cancer cells

Development of L-phenylalanine-grafted poly (vinyl alcohol) scaffold for selective capturing of cancer cells

Breast cancer is the most common cancer and the leading cause of cancer death in women. The majority of deaths from breast cancer do not result from the primary tumor itself but from metastasis to other organs in the body. Here, we report the development of a novel polymeric material that selectively traps breast cancer cells. The cancer cell-capturing polymer was synthesized by grafting L-phenylalanine onto a biocompatible poly(vinyl alcohol) (PVA-Phe). L-phenylalanine exhibits high affinity to L-type amino acid transporter 1 (LAT1), which is overexpressed on various cancer cells. We evaluated cancer cell-selective adhesion ability of PVA-Phe using MCF-7, a human hormone receptor-positive breast cancer model cell expressing LAT1. Our results suggest that LAT1 on cancer cells recognizes the L-phenylalanine and that MCF-7 selectively adheres to the synthesized PVA-Phe. For patients at risk of cancer recurrence, we expect that PVA-Phe synthesized in this study could serve to capture the remaining cancer cells when implanted in the body. Together with an appropriate scaffold that functions to attract cancer cells, PVA-Phe may have the potential to completely prevent cancer recurrence.

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来源期刊
Talanta Open
Talanta Open Chemistry-Analytical Chemistry
CiteScore
5.20
自引率
0.00%
发文量
86
审稿时长
49 days
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