单级自体软骨细胞联合植入透明质酸支架治疗膝关节软骨损伤:16例患者五年临床疗效的病例系列

Emmanouil T. Papakostas , Efthymios Papasoulis , Willem Cornelis de Jong , Aristotelis S. Sideridis , Argiris Karavelis , Konstantinos Epameinontidis , Ioannis P. Terzidis
{"title":"单级自体软骨细胞联合植入透明质酸支架治疗膝关节软骨损伤:16例患者五年临床疗效的病例系列","authors":"Emmanouil T. Papakostas ,&nbsp;Efthymios Papasoulis ,&nbsp;Willem Cornelis de Jong ,&nbsp;Aristotelis S. Sideridis ,&nbsp;Argiris Karavelis ,&nbsp;Konstantinos Epameinontidis ,&nbsp;Ioannis P. Terzidis","doi":"10.1016/j.jcjp.2024.100170","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>The direct reimplantation of autologous primary articular chondrocytes in a single-stage procedure for knee cartilage lesions is a novel approach, yet to be extensively reported. This study aimed to evaluate the clinical outcomes and reinterventions over a 5-year period post-surgery.</p></div><div><h3>Methods</h3><p>A prospective case series involving 16 patients (4 female, 12 male) with single or multiple focal knee cartilage lesions was conducted. The mean age at baseline was 36.8 (±11.5) years. The mean total lesion size was 4.5 (±2.3) cm<sup>2</sup> Patients underwent surgery where articular chondrocytes and mononuclear bone marrow cells were isolated, mixed, and seeded onto a hyaluronan-based scaffold within the lesion. A structured physical therapy regimen was followed, and patients were assessed using Knee injury and Osteoarthritis Outcome Score (KOOS) and International Knee Documentation Committee (IKDC) Subjective questionnaires at various intervals. Overall, patient-reported outcomes improved over the first 3 years post-surgery, with slight declines thereafter.</p></div><div><h3>Results</h3><p>Significant improvements over baseline were noted for various KOOS parameters and IKDC Subjective scores at different follow-up points. Reinterventions were required for two patients, one receiving intra-articular injections with mesenchymal stromal cells and another undergoing knee washout for septic arthritis while a meniscus implant was removed. Patient satisfaction at final follow-up was generally favorable. Despite a significant intraoperative cell isolation time of approximately 1.5 hours, the procedure demonstrated safety and efficacy, with an average of ±0.9 million articular chondrocytes obtained per case.</p></div><div><h3>Conclusions</h3><p>This study underscores the potential of coimplantation of intraoperatively isolated articular chondrocytes and bone marrow cells on a hyaluronan scaffold as a promising strategy for treating symptomatic knee cartilage lesions.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"4 2","pages":"Article 100170"},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254524000064/pdfft?md5=0ab2bdba63f5a05ef4eeabbe6b271be6&pid=1-s2.0-S2667254524000064-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Single-stage autologous chondrocyte coimplantation on a hyaluronan scaffold for the treatment of knee cartilage lesions: a case series of 16 patients with clinical outcomes up to 5 years\",\"authors\":\"Emmanouil T. Papakostas ,&nbsp;Efthymios Papasoulis ,&nbsp;Willem Cornelis de Jong ,&nbsp;Aristotelis S. Sideridis ,&nbsp;Argiris Karavelis ,&nbsp;Konstantinos Epameinontidis ,&nbsp;Ioannis P. Terzidis\",\"doi\":\"10.1016/j.jcjp.2024.100170\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>The direct reimplantation of autologous primary articular chondrocytes in a single-stage procedure for knee cartilage lesions is a novel approach, yet to be extensively reported. This study aimed to evaluate the clinical outcomes and reinterventions over a 5-year period post-surgery.</p></div><div><h3>Methods</h3><p>A prospective case series involving 16 patients (4 female, 12 male) with single or multiple focal knee cartilage lesions was conducted. The mean age at baseline was 36.8 (±11.5) years. The mean total lesion size was 4.5 (±2.3) cm<sup>2</sup> Patients underwent surgery where articular chondrocytes and mononuclear bone marrow cells were isolated, mixed, and seeded onto a hyaluronan-based scaffold within the lesion. A structured physical therapy regimen was followed, and patients were assessed using Knee injury and Osteoarthritis Outcome Score (KOOS) and International Knee Documentation Committee (IKDC) Subjective questionnaires at various intervals. Overall, patient-reported outcomes improved over the first 3 years post-surgery, with slight declines thereafter.</p></div><div><h3>Results</h3><p>Significant improvements over baseline were noted for various KOOS parameters and IKDC Subjective scores at different follow-up points. Reinterventions were required for two patients, one receiving intra-articular injections with mesenchymal stromal cells and another undergoing knee washout for septic arthritis while a meniscus implant was removed. Patient satisfaction at final follow-up was generally favorable. Despite a significant intraoperative cell isolation time of approximately 1.5 hours, the procedure demonstrated safety and efficacy, with an average of ±0.9 million articular chondrocytes obtained per case.</p></div><div><h3>Conclusions</h3><p>This study underscores the potential of coimplantation of intraoperatively isolated articular chondrocytes and bone marrow cells on a hyaluronan scaffold as a promising strategy for treating symptomatic knee cartilage lesions.</p></div>\",\"PeriodicalId\":100760,\"journal\":{\"name\":\"Journal of Cartilage & Joint Preservation\",\"volume\":\"4 2\",\"pages\":\"Article 100170\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667254524000064/pdfft?md5=0ab2bdba63f5a05ef4eeabbe6b271be6&pid=1-s2.0-S2667254524000064-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cartilage & Joint Preservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667254524000064\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cartilage & Joint Preservation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667254524000064","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

导言自体原发性关节软骨细胞直接再植入膝关节软骨病变的单阶段手术是一种新方法,目前尚未有广泛报道。本研究旨在评估术后 5 年的临床疗效和再干预情况。方法对 16 例单发或多发膝关节软骨病灶患者(女性 4 例,男性 12 例)进行了前瞻性病例系列研究。基线时的平均年龄为 36.8 (±11.5) 岁。病变总面积平均为 4.5 (±2.3) 平方厘米。患者接受手术治疗,分离、混合关节软骨细胞和单核骨髓细胞,并将其播种到病变部位的透明质酸基质支架上。患者接受了结构化物理治疗方案,并在不同时间间隔接受了膝关节损伤和骨关节炎结果评分(KOOS)和国际膝关节文献委员会(IKDC)主观问卷调查。结果在不同的随访时间点,KOOS 各项参数和 IKDC 主观评分均较基线有显著改善。两名患者需要重新进行干预,其中一名患者接受了间叶基质细胞的关节内注射,另一名患者因化脓性关节炎接受了膝关节冲洗,同时取出了半月板植入物。患者在最终随访中的满意度普遍良好。尽管术中细胞分离时间长达约1.5小时,但该手术显示出安全性和有效性,每例平均获得±90万个关节软骨细胞。结论这项研究强调了在透明质酸支架上共同植入术中分离的关节软骨细胞和骨髓细胞的潜力,认为这是治疗有症状的膝关节软骨损伤的一种有前途的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single-stage autologous chondrocyte coimplantation on a hyaluronan scaffold for the treatment of knee cartilage lesions: a case series of 16 patients with clinical outcomes up to 5 years

Introduction

The direct reimplantation of autologous primary articular chondrocytes in a single-stage procedure for knee cartilage lesions is a novel approach, yet to be extensively reported. This study aimed to evaluate the clinical outcomes and reinterventions over a 5-year period post-surgery.

Methods

A prospective case series involving 16 patients (4 female, 12 male) with single or multiple focal knee cartilage lesions was conducted. The mean age at baseline was 36.8 (±11.5) years. The mean total lesion size was 4.5 (±2.3) cm2 Patients underwent surgery where articular chondrocytes and mononuclear bone marrow cells were isolated, mixed, and seeded onto a hyaluronan-based scaffold within the lesion. A structured physical therapy regimen was followed, and patients were assessed using Knee injury and Osteoarthritis Outcome Score (KOOS) and International Knee Documentation Committee (IKDC) Subjective questionnaires at various intervals. Overall, patient-reported outcomes improved over the first 3 years post-surgery, with slight declines thereafter.

Results

Significant improvements over baseline were noted for various KOOS parameters and IKDC Subjective scores at different follow-up points. Reinterventions were required for two patients, one receiving intra-articular injections with mesenchymal stromal cells and another undergoing knee washout for septic arthritis while a meniscus implant was removed. Patient satisfaction at final follow-up was generally favorable. Despite a significant intraoperative cell isolation time of approximately 1.5 hours, the procedure demonstrated safety and efficacy, with an average of ±0.9 million articular chondrocytes obtained per case.

Conclusions

This study underscores the potential of coimplantation of intraoperatively isolated articular chondrocytes and bone marrow cells on a hyaluronan scaffold as a promising strategy for treating symptomatic knee cartilage lesions.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.90
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信