Legionella Pneumophila Infections among Egyptian Patients: Updated Diagnostic Tests and Evaluation

and q Real Time (RT) PCR for detection of L. pneumophila mip gene. Results: Prevalence of L. pneumophila in Zagazig university hospitals was 17% detected by L. pneumophila urinary antigen testing (LPUAT), 15% of samples were positive by q RT PCR based mip gene and 12 % positive by respiratory sample cultures. In comparison to respiratory sample culture, the gold standard, q RT PCR evaluation showed sensitivity 83.3%, specificity 94.3%, positive predictive value 66.7%, Negative predictive value 97.6% and accuracy of 93.0%. Conclusion: Molecular assays and UAT are promising methods that can be used in the rapid and early diagnosis of Legionella infections but they seem not to be sufficient to replace microbial cultures which must associate these techniques.