Aymen Aziz Khalid, Sarwa Azeez Khaled, H. S. Khuder
{"title":"揭示从尿路感染(UTI)患者体内分离出的大肠埃希菌的特异性凶猛基因","authors":"Aymen Aziz Khalid, Sarwa Azeez Khaled, H. S. Khuder","doi":"10.55544/jrasb.3.1.40","DOIUrl":null,"url":null,"abstract":"Background: The purpose of this research is to detect the occurrence of ferocity genes in uropathogenic E. coli that isolated from urinary tract infections by Polymerase chain reaction technique.\n Method: A whole of five strains of E.coli were obtained from patient with urinary tract infections. Identification of E.coli was depended on colonial morphology in culture media, gram stain, and conventional biochemical tests. ferocity factors genes (cnf-1, hly,urea A and ndv B) of E.coli strain were revelated by molecular technique.\nResults: ferocity genes were detected successfully by molecular technique in five isolates of E.coli, the predominance of genes in isolates are seen in percentage 100%\nConclusion: Urinary tract infections still the major universal infection in the worldwide. The one of the most prevalent infectious diseases induce renal failure in patients is UTI that generated by UPEC. The presence of ferocity factors are critical features where the grade of pathogenicity of UPEC strains relied on it. This study indicate that was a real relationship between the ferocity factor genes of E.coli and clinical symptoms of UTI.","PeriodicalId":507877,"journal":{"name":"Journal for Research in Applied Sciences and Biotechnology","volume":"3 3","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Revelation of Specific Ferocity Genes in Escherichia coli Isolated from Patient with Urinary Tract Infections (UTIs)\",\"authors\":\"Aymen Aziz Khalid, Sarwa Azeez Khaled, H. S. Khuder\",\"doi\":\"10.55544/jrasb.3.1.40\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: The purpose of this research is to detect the occurrence of ferocity genes in uropathogenic E. coli that isolated from urinary tract infections by Polymerase chain reaction technique.\\n Method: A whole of five strains of E.coli were obtained from patient with urinary tract infections. Identification of E.coli was depended on colonial morphology in culture media, gram stain, and conventional biochemical tests. ferocity factors genes (cnf-1, hly,urea A and ndv B) of E.coli strain were revelated by molecular technique.\\nResults: ferocity genes were detected successfully by molecular technique in five isolates of E.coli, the predominance of genes in isolates are seen in percentage 100%\\nConclusion: Urinary tract infections still the major universal infection in the worldwide. The one of the most prevalent infectious diseases induce renal failure in patients is UTI that generated by UPEC. The presence of ferocity factors are critical features where the grade of pathogenicity of UPEC strains relied on it. This study indicate that was a real relationship between the ferocity factor genes of E.coli and clinical symptoms of UTI.\",\"PeriodicalId\":507877,\"journal\":{\"name\":\"Journal for Research in Applied Sciences and Biotechnology\",\"volume\":\"3 3\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal for Research in Applied Sciences and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55544/jrasb.3.1.40\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal for Research in Applied Sciences and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55544/jrasb.3.1.40","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Revelation of Specific Ferocity Genes in Escherichia coli Isolated from Patient with Urinary Tract Infections (UTIs)
Background: The purpose of this research is to detect the occurrence of ferocity genes in uropathogenic E. coli that isolated from urinary tract infections by Polymerase chain reaction technique.
Method: A whole of five strains of E.coli were obtained from patient with urinary tract infections. Identification of E.coli was depended on colonial morphology in culture media, gram stain, and conventional biochemical tests. ferocity factors genes (cnf-1, hly,urea A and ndv B) of E.coli strain were revelated by molecular technique.
Results: ferocity genes were detected successfully by molecular technique in five isolates of E.coli, the predominance of genes in isolates are seen in percentage 100%
Conclusion: Urinary tract infections still the major universal infection in the worldwide. The one of the most prevalent infectious diseases induce renal failure in patients is UTI that generated by UPEC. The presence of ferocity factors are critical features where the grade of pathogenicity of UPEC strains relied on it. This study indicate that was a real relationship between the ferocity factor genes of E.coli and clinical symptoms of UTI.