有氧间歇训练与银杏叶补充剂对阿尔茨海默病大鼠被动回避记忆和 GDNF 基因表达的影响

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-13 DOI:10.5812/jjnpp-144050

Sepideh Basirat Dehkordi, Mehdi Mogharnasi, Majid Vahidian-Rezazadeh, M. Saravani

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引用次数: 0

摘要

背景：阿尔茨海默病（AD）是最常见的痴呆症，以记忆力减退为特征，是一种复杂的进行性神经退行性疾病。研究目的本研究旨在探讨有氧间歇训练（AIT）结合银杏叶（GB）补充剂对被动回避记忆功能和胶质细胞源性神经营养因子（GDNF）基因表达的影响。研究方法将 44 只雄性 Wistar 大鼠（每只体重约为 250 ± 20 克）饲养在特定温度的受控环境中。在为期 8 周（每周 5 天）的时间里，大鼠接受了 AIT 治疗，并服用了剂量为 100 毫克/千克的 GB 补充剂。大鼠被随机分为六组：健康对照组（HC）7 只；AD 对照组（AC）8 只；淀粉样蛋白-β（Aβ）注射加 GB 补充剂组（Aβ + GB）8 只；Aβ 注射加 GB 补充剂加 AIT 组（Aβ + GB + AIT）8 只；Aβ 注射加 AIT 组（Aβ + AIT）8 只；假手术组（SS）5 只。利用穿梭箱装置评估 Aβ 对被动回避记忆的影响，并确认疾病诱导。采用 qRT-PCR 方法检测 GDNF 基因表达。结果研究发现，在补充 AIT 和 GB 8 周后，与 AC 组相比，Aβ + AIT 组和 Aβ + GB + AIT 组的被动回避记忆有明显改善（分别为 P = 0.02 和 P = 0.000）。Aβ + GB + AIT 组的长期记忆能力明显增强（P = 0.000）。此外，与 AC 组相比，Aβ + GB + AIT 组和 Aβ + GB 组的 GDNF 表达明显增加（分别为 P = 0.000 和 P = 0.01）。结论观察到补充 AIT 和 GB 有协同作用，可导致 GDNF 基因表达增加，并改善 AD 雄性大鼠的被动回避记忆和长期记忆。

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Effects of Aerobic Interval Training Along with Ginkgo Biloba Supplementation on Passive Avoidance Memory and GDNF Gene Expression in Alzheimer Rats

Background: Alzheimer's disease (AD) represents the most prevalent form of dementia, characterized by memory loss, and is a complex, progressive neurodegenerative condition. Objectives: This study aimed to explore the impact of aerobic interval training (AIT) combined with Ginkgo biloba (GB) supplementation on passive avoidance memory function and the expression of the glial cell-derived neurotrophic factor (GDNF) gene in rats afflicted with AD. Methods: A total of forty-four male Wistar rats, each weighing approximately 250 ± 20 g, were maintained in a controlled environment at a specified temperature. Over 8 weeks (5 days per week), they underwent AIT and received GB supplements at a dosage of 100 mg/kg. The rats were randomly assigned into six groups: healthy control (HC) with 7 rats, AD control (AC) with 8 rats, amyloid-β (Aβ) injection plus GB supplement (Aβ + GB) with 8 rats, Aβ injection plus GB supplement plus AIT (Aβ + GB + AIT) with 8 rats, Aβ injection plus AIT (Aβ + AIT) with 8 rats, and sham surgery (SS) with 5 rats. The shuttlebox apparatus was utilized to assess the effect of Aβ on passive avoidance memory and to confirm disease induction. GDNF gene expression was measured using the qRT-PCR method. Results: The study found significant improvements in passive avoidance memory in the Aβ + AIT and Aβ + GB + AIT groups compared to the AC group after 8 weeks of AIT and GB supplementation (P = 0.02 and P = 0.000, respectively). Long-term memory performance was notably enhanced in the Aβ + GB + AIT group (P = 0.000). Additionally, GDNF expression significantly increased in the Aβ + GB + AIT and Aβ + GB groups in comparison to the AC group (P = 0.000 and P = 0.01, respectively). Conclusions: A synergistic effect of AIT and GB supplementation was observed, leading to an increase in GDNF gene expression and improvements in both passive avoidance memory and long-term memory in male rats with AD.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.