Examining the Proliferative Effect of Ozonated Olive Oil and Ozonated Distilled Water on Healthy Colon Fibroblast Cells and Colon Cancer Cells

Ozone therapy is an alternative form of treatment based on the administration of ozone gas to the body in the treatment of diseases and different medical conditions, and ozone applications are recommended in some studies. In this study, it was aimed to determine the possible anti-cancer activity by enriching extra virgin olive oil and distilled water with ozone gas and determining its cytotoxic effect on colon cancer and normal colon fibroblast cells. The effect of ozone enriched extra virgin olive oil and distilled water on cell viability was determined by MTT assay on DLD1 (colon cancer) and CCD-18Co (healthy colon fibroblast) cell lines. In the DLD-1 cell line, ozonated distilled water and olive oil decreased in vitro cell viability at all concentrations and this decrease was most pronounced at higher concentrations (5 and 10 ppm). In the CCD-18Co cell line, ozonized distilled water and ozonized olive oil increased in vitro cell viability at all concentrations, but this increase was not significant compared to the control. The results of this study are consistent with the results of other studies in the literature. Therefore, ozone therapy is thought to be promising in cancer treatment.