Van-Tuyen Le, Samuel Bertrand, Marion Brandolini‑Bunlon, Emmanuel Gentil, T. Robiou du Pont, Vony Rabesaotra, G. Wielgosz-Collin, Aurélie Mossion, O. Grovel
{"title":"海洋来源的限制青霉在环境相关条件下引起的全局代谢组变化","authors":"Van-Tuyen Le, Samuel Bertrand, Marion Brandolini‑Bunlon, Emmanuel Gentil, T. Robiou du Pont, Vony Rabesaotra, G. Wielgosz-Collin, Aurélie Mossion, O. Grovel","doi":"10.5802/crchim.276","DOIUrl":null,"url":null,"abstract":"Marine fungi have been found in all habitats and are able to adapt to their environmental niche conditions. In this study, a combination of LC-HRMS and GC-MS analytical approaches was used to analyse the whole metabolic changes of a marine sourced Penicillium restrictum strain isolated from a marine shellfish area. The P. restrictum MMS417 strain was grown on seven different media including an ecological one with two different water sources (synthetic sea water and distilled water) conditions following the OSMAC approach. Extracts of all media were analysed by LC-HRMS (lipids and specialised metabolites profiling) and GC-MS (fatty acids profiling). Aquired data were analysed using a multiblock strategy to highlight metabolic modification in regards to water conditions and to environmentally relevant conditions (mussel-based culture medium). This revealed that fatty acid composition of lipids was the most altered part of the explored metabolisms either looking to water effect and to environmentally relevant conditions. In particular, data showed that P. re-strictum MMS417 is able to produce lipids that include fatty acids usually produced by the mussel itself. This study also provides insight into the P. restrictum adaptation to marine salinity through fatty acids alteration. and shows that lipid metabolisms if far more altered in an OSMAC approach than the specialized metabolism. This study finally highlights the need for using environnement-mimicking culture conditions to reveal the metabolic potentialities of marine microbes.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":" 42","pages":""},"PeriodicalIF":16.4000,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum\",\"authors\":\"Van-Tuyen Le, Samuel Bertrand, Marion Brandolini‑Bunlon, Emmanuel Gentil, T. Robiou du Pont, Vony Rabesaotra, G. Wielgosz-Collin, Aurélie Mossion, O. Grovel\",\"doi\":\"10.5802/crchim.276\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Marine fungi have been found in all habitats and are able to adapt to their environmental niche conditions. In this study, a combination of LC-HRMS and GC-MS analytical approaches was used to analyse the whole metabolic changes of a marine sourced Penicillium restrictum strain isolated from a marine shellfish area. The P. restrictum MMS417 strain was grown on seven different media including an ecological one with two different water sources (synthetic sea water and distilled water) conditions following the OSMAC approach. Extracts of all media were analysed by LC-HRMS (lipids and specialised metabolites profiling) and GC-MS (fatty acids profiling). Aquired data were analysed using a multiblock strategy to highlight metabolic modification in regards to water conditions and to environmentally relevant conditions (mussel-based culture medium). This revealed that fatty acid composition of lipids was the most altered part of the explored metabolisms either looking to water effect and to environmentally relevant conditions. In particular, data showed that P. re-strictum MMS417 is able to produce lipids that include fatty acids usually produced by the mussel itself. This study also provides insight into the P. restrictum adaptation to marine salinity through fatty acids alteration. and shows that lipid metabolisms if far more altered in an OSMAC approach than the specialized metabolism. 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Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum
Marine fungi have been found in all habitats and are able to adapt to their environmental niche conditions. In this study, a combination of LC-HRMS and GC-MS analytical approaches was used to analyse the whole metabolic changes of a marine sourced Penicillium restrictum strain isolated from a marine shellfish area. The P. restrictum MMS417 strain was grown on seven different media including an ecological one with two different water sources (synthetic sea water and distilled water) conditions following the OSMAC approach. Extracts of all media were analysed by LC-HRMS (lipids and specialised metabolites profiling) and GC-MS (fatty acids profiling). Aquired data were analysed using a multiblock strategy to highlight metabolic modification in regards to water conditions and to environmentally relevant conditions (mussel-based culture medium). This revealed that fatty acid composition of lipids was the most altered part of the explored metabolisms either looking to water effect and to environmentally relevant conditions. In particular, data showed that P. re-strictum MMS417 is able to produce lipids that include fatty acids usually produced by the mussel itself. This study also provides insight into the P. restrictum adaptation to marine salinity through fatty acids alteration. and shows that lipid metabolisms if far more altered in an OSMAC approach than the specialized metabolism. This study finally highlights the need for using environnement-mimicking culture conditions to reveal the metabolic potentialities of marine microbes.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.