Simone Baldi, Alessio Fabbrizzi, Leandro Di Gloria, Marco Pallecchi, Giulia Nannini, Mario D'Ambrosio, Cristina Luceri, Gianluca Bartolucci, Matteo Ramazzotti, Giovanni Fontana, Claudia Mannini, Federico Lavorini, Amedeo Amedei
{"title":"人类难治性慢性咳嗽发病机制中微生物肠肺轴的改变初探","authors":"Simone Baldi, Alessio Fabbrizzi, Leandro Di Gloria, Marco Pallecchi, Giulia Nannini, Mario D'Ambrosio, Cristina Luceri, Gianluca Bartolucci, Matteo Ramazzotti, Giovanni Fontana, Claudia Mannini, Federico Lavorini, Amedeo Amedei","doi":"10.1007/s00408-024-00681-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Cough represents a natural mechanism that plays an important defensive role in the respiratory tract, but in some conditions, it may become persistent, nonproductive, and harmful. In general, refractory chronic cough (RCC) occurs in about 20% of individuals; hence, we aimed to assess the presence of altered gut-lung communication in RCC patients through a compositional and functional characterization of both gut (GM) and oral microbiota (OM).</p><p><strong>Methods: </strong>16S rRNA sequencing was used to characterize both GM and OM composition of RCC patients and healthy controls (HC). PICRUST2 assessed functional changes in microbial communities while gas chromatography was used to evaluate fecal short-chain fatty acid levels and serum-free fatty acid (FFA) abundances.</p><p><strong>Results: </strong>In comparison with HC, RCC patients reported increased saliva alpha-diversity and statistically significant beta-diversity in both GM and OM. Also, a, respectively, significant increased or reduced Firmicutes/Bacteroidota ratio in stool and saliva samples of RCC patients has been shown, in addition to a modification of the abundances of several taxa in both GM and OM. Moreover, a potential fecal over-expression of lipopolysaccharide biosynthesis and lipoic acid metabolism pathways and several differences in serum FFA levels have been reported in RCC patients than in HC.</p><p><strong>Conclusion: </strong>Since differences in both GM and OM of RCC patients have been documented, these findings could provide new information about RCC pathogenesis and also pave the way for the development of novel nutritional or pharmacological interventions for the management of RCC through the restoration of eubiotic gut-lung communication.</p>","PeriodicalId":18163,"journal":{"name":"Lung","volume":" ","pages":"107-118"},"PeriodicalIF":4.6000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11009740/pdf/","citationCount":"0","resultStr":"{\"title\":\"First Exploration of the Altered Microbial Gut-Lung Axis in the Pathogenesis of Human Refractory Chronic Cough.\",\"authors\":\"Simone Baldi, Alessio Fabbrizzi, Leandro Di Gloria, Marco Pallecchi, Giulia Nannini, Mario D'Ambrosio, Cristina Luceri, Gianluca Bartolucci, Matteo Ramazzotti, Giovanni Fontana, Claudia Mannini, Federico Lavorini, Amedeo Amedei\",\"doi\":\"10.1007/s00408-024-00681-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Cough represents a natural mechanism that plays an important defensive role in the respiratory tract, but in some conditions, it may become persistent, nonproductive, and harmful. In general, refractory chronic cough (RCC) occurs in about 20% of individuals; hence, we aimed to assess the presence of altered gut-lung communication in RCC patients through a compositional and functional characterization of both gut (GM) and oral microbiota (OM).</p><p><strong>Methods: </strong>16S rRNA sequencing was used to characterize both GM and OM composition of RCC patients and healthy controls (HC). PICRUST2 assessed functional changes in microbial communities while gas chromatography was used to evaluate fecal short-chain fatty acid levels and serum-free fatty acid (FFA) abundances.</p><p><strong>Results: </strong>In comparison with HC, RCC patients reported increased saliva alpha-diversity and statistically significant beta-diversity in both GM and OM. Also, a, respectively, significant increased or reduced Firmicutes/Bacteroidota ratio in stool and saliva samples of RCC patients has been shown, in addition to a modification of the abundances of several taxa in both GM and OM. Moreover, a potential fecal over-expression of lipopolysaccharide biosynthesis and lipoic acid metabolism pathways and several differences in serum FFA levels have been reported in RCC patients than in HC.</p><p><strong>Conclusion: </strong>Since differences in both GM and OM of RCC patients have been documented, these findings could provide new information about RCC pathogenesis and also pave the way for the development of novel nutritional or pharmacological interventions for the management of RCC through the restoration of eubiotic gut-lung communication.</p>\",\"PeriodicalId\":18163,\"journal\":{\"name\":\"Lung\",\"volume\":\" \",\"pages\":\"107-118\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11009740/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lung\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00408-024-00681-7\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"RESPIRATORY SYSTEM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lung","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00408-024-00681-7","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/25 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
引用次数: 0
摘要
目的:咳嗽是一种自然机制,在呼吸道中发挥着重要的防御作用,但在某些情况下,咳嗽可能会变得持续、无益且有害。一般来说,约有 20% 的人患有难治性慢性咳嗽 (RCC);因此,我们旨在通过对肠道 (GM) 和口腔微生物群 (OM) 的组成和功能特性进行分析,评估 RCC 患者的肠道-肺部沟通是否发生了改变。PICRUST2评估了微生物群落的功能变化,而气相色谱法则用于评估粪便短链脂肪酸水平和血清游离脂肪酸(FFA)丰度:结果:与白血病患者相比,RCC 患者的唾液α-多样性增加,GM 和 OM 的β-多样性也有显著的统计学意义。此外,RCC 患者粪便和唾液样本中的固着菌/类菌比例也分别出现了显著的增高或降低,此外,GM 和 OM 中多个分类群的丰度也发生了变化。此外,据报道,RCC 患者粪便中脂多糖生物合成和硫辛酸代谢途径可能过度表达,血清中的 FFA 水平也与 HC 患者存在差异:结论:由于 RCC 患者的 GM 和 OM 均存在差异,这些发现可为 RCC 的发病机制提供新的信息,并为开发新型营养或药物干预措施铺平道路,从而通过恢复肠肺的生态沟通来治疗 RCC。
First Exploration of the Altered Microbial Gut-Lung Axis in the Pathogenesis of Human Refractory Chronic Cough.
Purpose: Cough represents a natural mechanism that plays an important defensive role in the respiratory tract, but in some conditions, it may become persistent, nonproductive, and harmful. In general, refractory chronic cough (RCC) occurs in about 20% of individuals; hence, we aimed to assess the presence of altered gut-lung communication in RCC patients through a compositional and functional characterization of both gut (GM) and oral microbiota (OM).
Methods: 16S rRNA sequencing was used to characterize both GM and OM composition of RCC patients and healthy controls (HC). PICRUST2 assessed functional changes in microbial communities while gas chromatography was used to evaluate fecal short-chain fatty acid levels and serum-free fatty acid (FFA) abundances.
Results: In comparison with HC, RCC patients reported increased saliva alpha-diversity and statistically significant beta-diversity in both GM and OM. Also, a, respectively, significant increased or reduced Firmicutes/Bacteroidota ratio in stool and saliva samples of RCC patients has been shown, in addition to a modification of the abundances of several taxa in both GM and OM. Moreover, a potential fecal over-expression of lipopolysaccharide biosynthesis and lipoic acid metabolism pathways and several differences in serum FFA levels have been reported in RCC patients than in HC.
Conclusion: Since differences in both GM and OM of RCC patients have been documented, these findings could provide new information about RCC pathogenesis and also pave the way for the development of novel nutritional or pharmacological interventions for the management of RCC through the restoration of eubiotic gut-lung communication.
期刊介绍:
Lung publishes original articles, reviews and editorials on all aspects of the healthy and diseased lungs, of the airways, and of breathing. Epidemiological, clinical, pathophysiological, biochemical, and pharmacological studies fall within the scope of the journal. Case reports, short communications and technical notes can be accepted if they are of particular interest.