Bletilla striata 多糖(BSP)和壳聚糖(CS)复合止血海绵(BSP-CS)的工艺优化和特性评估。

IF 1.6 4区 医学 Q4 BIOPHYSICS
Biointerphases Pub Date : 2024-03-01 DOI:10.1116/6.0003369
Yeshan Zhang, Xue Han, Jun Zhao, Menglan Gan, Yaya Chen, Jinxia Zhang, Yu He, Mingkai Wu, Hai Liu
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引用次数: 0

摘要

用草酰氯对条纹紫苏多糖(BSP)和壳聚糖(CS)进行化学交联,制备出一种复合止血海绵(BSP-CS),并采用箱-贝肯设计(BBD)和响应面方法对工艺参数进行了优化。为了优化止血海绵的性能,我们调整了自变量的比例、草酰氯的添加量和冻干体积。我们对 BSP-CS 的止血适用性进行了一系列评估。表征结果显示,BSP-CS 在 72 小时内对大肠杆菌、金黄色葡萄球菌和绿脓杆菌具有稳定的抑菌作用,抑菌率在 30% 以上。CCK-8 细胞毒性试验表明,BSP-CS 对 L929 细胞的增殖有一定的促进作用。在小鼠断尾实验中,BSP-CS 的止血时间为(463.0±38.16)s,与纱布组的(554.3±34.67)s 相比,平均缩短了 91.3s。BSP-CS 组的失血量为 28.47±3.74 毫克,比对照纱布组(43.6±3.83 毫克)低 34.7%。在体外凝血实验中,BSP-CS 组的体外凝血指数为 97.29%±1.8%,低于对照组的 8.6%。BSP-CS 组的 CT 值为 240±15 s,比纱布组(355±31.22 s)低 155 s。所有表征结果表明,BSP-CS 是一种出色的止血材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Process optimization and character evaluation of Bletilla striata polysaccharide (BSP) and chitosan (CS) composite hemostatic sponge (BSP-CS).

Bletilla striata polysaccharide (BSP) and chitosan (CS) were chemically cross-linked using oxalyl chloride to prepare a composite hemostatic sponge (BSP-CS), and the process parameters were optimized using the Box-Behnken design (BBD) with response surface methodology. To optimize the performance of the hemostatic sponge, we adjusted the ratio of independent variables, the amount of oxalyl chloride added, and the freeze-dried volume. A series of evaluations were conducted on the hemostatic applicability of BSP-CS. The characterization results revealed that BSP-CS had a stable bacteriostatic effect on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa within 72 h, and the bacteriostatic rate was above 30%. The CCK-8 cytotoxicity test demonstrated that BSP-CS had a certain effect on promoting cell proliferation of L929 cells. In the mouse tail-cutting experiment, the hemostasis time of BSP-CS was 463.0±38.16 s, shortened by 91.3 s on average compared with 554.3±34.67 s of the gauze group. The blood loss of the BSP-CS group was 28.47±3.74 mg, which was 34.7% lower than that of the control gauze group (43.6±3.83 mg). In the in vitro coagulation experiment, the in vitro coagulation index of the BSP-CS group was 97.29%±1.8%, which was reduced to 8.6% of the control group. The CT value of the BSP-CS group was 240±15 s, which was 155 s lower than that of the gauze group (355±31.22 s). All characterization results indicate that BSP-CS is an excellent hemostatic material.

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来源期刊
Biointerphases
Biointerphases 生物-材料科学:生物材料
自引率
0.00%
发文量
35
期刊介绍: Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.
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