从 Actinoplanes deccanensis 中分离出菲达霉素和分流代谢物

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-20 DOI:10.1002/hlca.202400013

Erik Jung, Dr. Maja Hunter, Dr. Andrea Dorst, Alexander Major, Tatjana Teofilovic, Prof. Dr. Rolf Müller, Prof. Dr. Karl Gademann

{"title":"从 Actinoplanes deccanensis 中分离出菲达霉素和分流代谢物","authors":"Erik Jung, Dr. Maja Hunter, Dr. Andrea Dorst, Alexander Major, Tatjana Teofilovic, Prof. Dr. Rolf Müller, Prof. Dr. Karl Gademann","doi":"10.1002/hlca.202400013","DOIUrl":null,"url":null,"abstract":"A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from Actinoplanes deccanensis and the isolation of shunt metabolites from A. deccanensis fdxG2− is reported. We constructed the mutant strain A. deccanensis fdxG2− by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex A. deccanensis fdxG2− extracts into the single compound FdxG2-OH via methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/hlca.202400013","citationCount":"0","resultStr":"{\"title\":\"Isolation of Fidaxomicin and Shunt Metabolites from Actinoplanes deccanensis\",\"authors\":\"Erik Jung, Dr. Maja Hunter, Dr. Andrea Dorst, Alexander Major, Tatjana Teofilovic, Prof. Dr. Rolf Müller, Prof. Dr. Karl Gademann\",\"doi\":\"10.1002/hlca.202400013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from Actinoplanes deccanensis and the isolation of shunt metabolites from A. deccanensis fdxG2− is reported. We constructed the mutant strain A. deccanensis fdxG2− by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex A. deccanensis fdxG2− extracts into the single compound FdxG2-OH via methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/hlca.202400013\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/hlca.202400013\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/hlca.202400013","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

报告了从 Actinoplanes deccanensis 分离抗生素 fidaxomicin（Fdx）以及从 A. deccanensisfdxG2- 分离分流代谢物的方案。我们通过遗传操作构建了突变株 A. deccanensisfdxG2-，从而能够分离出分流代谢产物，作为 Fdx 半合成类似物的有用起点。此外，还介绍了通过甲醇分解将复杂的 A. deccanensisfdxG2- 提取物转化为单一化合物 FdxG2-OH 的合成方案。该合成过程附有图像和实用说明。SI 中给出了完整的结构分配，并公布了表征数据文件，以帮助实验人员。该方案也适合作为本科生的实验室项目。我们希望通过提供这一程序来促进新 Fdx 衍生物的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Isolation of Fidaxomicin and Shunt Metabolites from Actinoplanes deccanensis

查看原文本刊更多论文

Isolation of Fidaxomicin and Shunt Metabolites from Actinoplanes deccanensis

A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from Actinoplanes deccanensis and the isolation of shunt metabolites from A. deccanensis fdxG2⁻ is reported. We constructed the mutant strain A. deccanensis fdxG2⁻ by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex A. deccanensis fdxG2⁻ extracts into the single compound FdxG2-OH via methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.