Erik Jung, Dr. Maja Hunter, Dr. Andrea Dorst, Alexander Major, Tatjana Teofilovic, Prof. Dr. Rolf Müller, Prof. Dr. Karl Gademann
{"title":"从 Actinoplanes deccanensis 中分离出菲达霉素和分流代谢物","authors":"Erik Jung, Dr. Maja Hunter, Dr. Andrea Dorst, Alexander Major, Tatjana Teofilovic, Prof. Dr. Rolf Müller, Prof. Dr. Karl Gademann","doi":"10.1002/hlca.202400013","DOIUrl":null,"url":null,"abstract":"<p>A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from <i>Actinoplanes deccanensis</i> and the isolation of shunt metabolites from <i>A. deccanensis fdxG2</i><sup><i>−</i></sup> is reported. We constructed the mutant strain <i>A. deccanensis fdxG2</i><sup><i>−</i></sup> by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex <i>A. deccanensis fdxG2</i><sup><i>−</i></sup> extracts into the single compound FdxG2-OH <i>via</i> methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.</p>","PeriodicalId":12842,"journal":{"name":"Helvetica Chimica Acta","volume":"107 5","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/hlca.202400013","citationCount":"0","resultStr":"{\"title\":\"Isolation of Fidaxomicin and Shunt Metabolites from Actinoplanes deccanensis\",\"authors\":\"Erik Jung, Dr. Maja Hunter, Dr. Andrea Dorst, Alexander Major, Tatjana Teofilovic, Prof. Dr. Rolf Müller, Prof. Dr. Karl Gademann\",\"doi\":\"10.1002/hlca.202400013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from <i>Actinoplanes deccanensis</i> and the isolation of shunt metabolites from <i>A. deccanensis fdxG2</i><sup><i>−</i></sup> is reported. We constructed the mutant strain <i>A. deccanensis fdxG2</i><sup><i>−</i></sup> by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex <i>A. deccanensis fdxG2</i><sup><i>−</i></sup> extracts into the single compound FdxG2-OH <i>via</i> methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.</p>\",\"PeriodicalId\":12842,\"journal\":{\"name\":\"Helvetica Chimica Acta\",\"volume\":\"107 5\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/hlca.202400013\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Helvetica Chimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/hlca.202400013\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Helvetica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/hlca.202400013","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
报告了从 Actinoplanes deccanensis 分离抗生素 fidaxomicin（Fdx）以及从 A. deccanensisfdxG2- 分离分流代谢物的方案。我们通过遗传操作构建了突变株 A. deccanensisfdxG2-，从而能够分离出分流代谢产物，作为 Fdx 半合成类似物的有用起点。此外，还介绍了通过甲醇分解将复杂的 A. deccanensisfdxG2- 提取物转化为单一化合物 FdxG2-OH 的合成方案。该合成过程附有图像和实用说明。SI 中给出了完整的结构分配，并公布了表征数据文件，以帮助实验人员。该方案也适合作为本科生的实验室项目。我们希望通过提供这一程序来促进新 Fdx 衍生物的研究。
Isolation of Fidaxomicin and Shunt Metabolites from Actinoplanes deccanensis
A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from Actinoplanes deccanensis and the isolation of shunt metabolites from A. deccanensis fdxG2− is reported. We constructed the mutant strain A. deccanensis fdxG2− by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex A. deccanensis fdxG2− extracts into the single compound FdxG2-OH via methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.
期刊介绍：
Helvetica Chimica Acta, founded by the Swiss Chemical Society in 1917, is a monthly multidisciplinary journal dedicated to the dissemination of knowledge in all disciplines of chemistry (organic, inorganic, physical, technical, theoretical and analytical chemistry) as well as research at the interface with other sciences, where molecular aspects are key to the findings. Helvetica Chimica Acta is committed to the publication of original, high quality papers at the frontier of scientific research. All contributions will be peer reviewed with the highest possible standards and published within 3 months of receipt, with no restriction on the length of the papers and in full color.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
