PURPL 通过调节 RBM4/xCT 信号促进肺癌中 M2 巨噬细胞的极化

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-03-01 DOI:10.1615/critreveukaryotgeneexpr.2024052788

Jipeng Guo, Chongwen Gong, Hao Wang

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引用次数: 0

摘要

肺癌是全球最常见的恶性肿瘤。长非编码 RNA（lncRNA）PURPL 在多种癌症中出现异常。然而，有关其在肺癌中作用的报道却很有限。本研究旨在探讨lncRNA PURPL在肺癌中的潜在作用。研究采用 RT-qPCR 方法测定 PURPL 和 mRNA 的表达。使用 RNA FISH 检测 PURPL 的位置。使用 Western 印迹检测蛋白质表达。使用流式细胞术测定细胞功能。使用 RIP 试验证实了 PURPL 和 RBM4 之间的相互作用。PURPL在肺癌细胞和患者体内过表达。过表达的PURPL可促进M2巨噬细胞极化并抑制铁变态反应。此外，PURPL通过调节RBM4维持xCT的mRNA稳定性。敲除xCT可拮抗过表达PURPL的影响，并通过诱导巨噬细胞铁嗜性抑制M2巨噬细胞极化。PURPL/RBM4/xCT轴促进了肺癌中M2巨噬细胞的极化。因此，PURPL可能是肺癌的潜在靶点。

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PURPL promotes M2 macrophage polarization in lung cancer via regulating RBM4/xCT signaling

Lung cancer is the most common malignancy worldwide. Long non-coding RNA (lncRNA) PURPL is abnormally in various cancers. However, the reports on its roles in lung cancer are limited. The purpose of present study is to investigate the potentials of lncRNA PURPL in lung cancer. PURPL and mRNA expression was determined using RT-qPCR. The location of PURPL was detected using RNA FISH assay. Protein expression was detected using western blot. Cellular functions were determined using flow cytometry. The interaction between PURPL and RBM4 was confirmed using RIP assay. PURPL was overexpressed in lung cancer cells and patients. Overexpressed PURPL promoted M2 macrophage polarization and suppressed ferroptosis. Additionally, PURPL maintained the mRNA stability of xCT via regulating RBM4. xCT knockdown antagonized the effects of overexpressed PURPL and inhibited M2 macrophage polarization via inducing macrophage ferroptosis. PURPL/RBM4/xCT axis promoted M2 macrophage polarization in lung cancer. Therefore, PURPL may be a potential target of lung cancer.

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.