通过分子内环化作用生成含氟聚甲基染料

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology Pub Date : 2024-03-23 DOI:10.1016/j.cbpa.2024.102444

Annabell Martin , Pablo Rivera-Fuentes

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引用次数: 0

摘要

荧光成像在生物过程研究中起着举足轻重的作用，而细胞渗透性荧光染料对于高特异性地观察细胞内结构至关重要。多聚甲醛染料是单分子定位显微镜和活体成像中极其重要的荧光团，但它们在活细胞中的应用一直受到高背景荧光和低膜渗透性的限制。在这篇综述中，我们总结了通过分子内环化技术开发致氟聚甲醛染料的最新进展。最后，我们展望了含氟多亚甲基染料在生物成像中的应用前景。

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Fluorogenic polymethine dyes by intramolecular cyclization

Fluorescence imaging plays a pivotal role in the study of biological processes, and cell-permeable fluorogenic dyes are crucial to visualize intracellular structures with high specificity. Polymethine dyes are vitally important fluorophores in single-molecule localization microscopy and in vivo imaging, but their use in live cells has been limited by high background fluorescence and low membrane permeability. In this review, we summarize recent advances in the development of fluorogenic polymethine dyes via intramolecular cyclization. Finally, we offer an outlook on the prospects of fluorogenic polymethine dyes for bioimaging.

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来源期刊

Current Opinion in Chemical Biology 生物-生化与分子生物学

CiteScore

13.30

自引率

1.30%

发文量

113

审稿时长

74 days

期刊介绍： COCHBI (Current Opinion in Chemical Biology) is a systematic review journal designed to offer specialists a unique and educational platform. Its goal is to help professionals stay informed about the growing volume of information in the field of Chemical Biology through systematic reviews.