{"title":"接穗和砧木切口部位以及科内和科间嫁接接头的植物激素分析。","authors":"Kohei Kawaguchi, Michitaka Notaguchi, Koji Okayasu, Yu Sawai, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Shungo Otagaki, Shogo Matsumoto, Katsuhiro Shiratake","doi":"10.1080/15592324.2024.2331358","DOIUrl":null,"url":null,"abstract":"<p><p>Many previous studies have suggested that various plant hormones play essential roles in the grafting process. In this study, to understand the plant hormones that accumulate in the graft junctions, whether these are supplied from the scion or rootstock, and how these hormones play a role in the grafting process, we performed a hormonome analysis that accumulated in the incision site of the upper plants from the incision as \"ungrafted scion\" and lower plants from the incision as \"ungrafted rootstock\" in <i>Nicotiana benthamiana</i>. The results revealed that indole-3-acetic acid (IAA) and gibberellic acid (GA), which regulate cell division; abscisic acid (ABA) and jasmonic acid (JA), which regulate xylem formation; cytokinin (CK), which regulates callus formation, show different accumulation patterns in the incision sites of the ungrafted scion and rootstock. In addition, to try discussing the differences in the degree and speed of each event during the grafting process between intra- and inter-family grafting by determining the concentration and accumulation timing of plant hormones in the graft junctions, we performed hormonome analysis of graft junctions of intra-family grafted plants with <i>N. benthamiana</i> as scion and <i>Solanum lycopersicum</i> as rootstock (<i>Nb/Sl</i>) and inter-family grafted plants with <i>N. benthamiana</i> as scion and <i>Arabidopsis thaliana</i> as rootstock (<i>Nb/At</i>), using the ability of <i>Nicotiana</i> species to graft with many plant species. The results revealed that ABA and CK showed different accumulation timings; IAA, JA, and salicylic acid (SA) showed similar accumulation timings, while different accumulated concentrations in the graft junctions of <i>Nb/Sl</i> and <i>Nb/At</i>. This information is important for understanding the molecular mechanisms of plant hormones in the grafting process and the differences in molecular mechanisms between intra- and inter-family grafting.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2331358"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962582/pdf/","citationCount":"0","resultStr":"{\"title\":\"Plant hormone profiling of scion and rootstock incision sites and intra- and inter-family graft junctions in <i>Nicotiana benthamiana</i>.\",\"authors\":\"Kohei Kawaguchi, Michitaka Notaguchi, Koji Okayasu, Yu Sawai, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Shungo Otagaki, Shogo Matsumoto, Katsuhiro Shiratake\",\"doi\":\"10.1080/15592324.2024.2331358\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Many previous studies have suggested that various plant hormones play essential roles in the grafting process. In this study, to understand the plant hormones that accumulate in the graft junctions, whether these are supplied from the scion or rootstock, and how these hormones play a role in the grafting process, we performed a hormonome analysis that accumulated in the incision site of the upper plants from the incision as \\\"ungrafted scion\\\" and lower plants from the incision as \\\"ungrafted rootstock\\\" in <i>Nicotiana benthamiana</i>. The results revealed that indole-3-acetic acid (IAA) and gibberellic acid (GA), which regulate cell division; abscisic acid (ABA) and jasmonic acid (JA), which regulate xylem formation; cytokinin (CK), which regulates callus formation, show different accumulation patterns in the incision sites of the ungrafted scion and rootstock. In addition, to try discussing the differences in the degree and speed of each event during the grafting process between intra- and inter-family grafting by determining the concentration and accumulation timing of plant hormones in the graft junctions, we performed hormonome analysis of graft junctions of intra-family grafted plants with <i>N. benthamiana</i> as scion and <i>Solanum lycopersicum</i> as rootstock (<i>Nb/Sl</i>) and inter-family grafted plants with <i>N. benthamiana</i> as scion and <i>Arabidopsis thaliana</i> as rootstock (<i>Nb/At</i>), using the ability of <i>Nicotiana</i> species to graft with many plant species. The results revealed that ABA and CK showed different accumulation timings; IAA, JA, and salicylic acid (SA) showed similar accumulation timings, while different accumulated concentrations in the graft junctions of <i>Nb/Sl</i> and <i>Nb/At</i>. This information is important for understanding the molecular mechanisms of plant hormones in the grafting process and the differences in molecular mechanisms between intra- and inter-family grafting.</p>\",\"PeriodicalId\":94172,\"journal\":{\"name\":\"Plant signaling & behavior\",\"volume\":\"19 1\",\"pages\":\"2331358\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962582/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant signaling & behavior\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/15592324.2024.2331358\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant signaling & behavior","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15592324.2024.2331358","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/21 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
以往的许多研究表明,各种植物激素在嫁接过程中发挥着重要作用。在本研究中,为了了解在嫁接接合部积累的植物激素,这些激素是由接穗还是砧木提供,以及这些激素如何在嫁接过程中发挥作用,我们对从切口作为 "未嫁接接穗 "的上部植株和从切口作为 "未嫁接砧木 "的下部植株的切口部位积累的激素组进行了分析。结果发现,调节细胞分裂的吲哚-3-乙酸(IAA)和赤霉素(GA)、调节木质部形成的脱落酸(ABA)和茉莉酸(JA)、调节胼胝体形成的细胞分裂素(CK)在未嫁接接穗和砧木的切口部位呈现出不同的积累模式。此外,为了通过测定嫁接接合部植物激素的浓度和积累时间来探讨科内嫁接和科间嫁接过程中各事件发生的程度和速度差异,我们对以 N. benthamiana 为接穗和砧木的科内嫁接植株的嫁接接合部进行了激素组分析。我们利用烟碱类植物能与多种植物嫁接的特性,对以 N. benthamiana 为接穗、Solanum lycopersicum 为砧木(Nb/Sl)的科内嫁接植物和以 N. benthamiana 为接穗、拟南芥为砧木(Nb/At)的科间嫁接植物的嫁接接头进行了激素组分析。结果发现,ABA和CK的积累时间不同;IAA、JA和水杨酸(SA)的积累时间相似,但在Nb/Sl和Nb/At的嫁接接头处的积累浓度不同。这些信息对于了解植物激素在嫁接过程中的分子机制以及科内嫁接和科间嫁接在分子机制上的差异具有重要意义。
Plant hormone profiling of scion and rootstock incision sites and intra- and inter-family graft junctions in Nicotiana benthamiana.
Many previous studies have suggested that various plant hormones play essential roles in the grafting process. In this study, to understand the plant hormones that accumulate in the graft junctions, whether these are supplied from the scion or rootstock, and how these hormones play a role in the grafting process, we performed a hormonome analysis that accumulated in the incision site of the upper plants from the incision as "ungrafted scion" and lower plants from the incision as "ungrafted rootstock" in Nicotiana benthamiana. The results revealed that indole-3-acetic acid (IAA) and gibberellic acid (GA), which regulate cell division; abscisic acid (ABA) and jasmonic acid (JA), which regulate xylem formation; cytokinin (CK), which regulates callus formation, show different accumulation patterns in the incision sites of the ungrafted scion and rootstock. In addition, to try discussing the differences in the degree and speed of each event during the grafting process between intra- and inter-family grafting by determining the concentration and accumulation timing of plant hormones in the graft junctions, we performed hormonome analysis of graft junctions of intra-family grafted plants with N. benthamiana as scion and Solanum lycopersicum as rootstock (Nb/Sl) and inter-family grafted plants with N. benthamiana as scion and Arabidopsis thaliana as rootstock (Nb/At), using the ability of Nicotiana species to graft with many plant species. The results revealed that ABA and CK showed different accumulation timings; IAA, JA, and salicylic acid (SA) showed similar accumulation timings, while different accumulated concentrations in the graft junctions of Nb/Sl and Nb/At. This information is important for understanding the molecular mechanisms of plant hormones in the grafting process and the differences in molecular mechanisms between intra- and inter-family grafting.