Arfah Naveed Dar , Javeria Shahzad , Joham Sarfraz Ali , Usama Sarwar , Anila Sajjad , Muhammad Zia
{"title":"生物测定指导下的三萜分离及其生物评估--使用一种重要药用植物的枝条提取物","authors":"Arfah Naveed Dar , Javeria Shahzad , Joham Sarfraz Ali , Usama Sarwar , Anila Sajjad , Muhammad Zia","doi":"10.1016/j.prenap.2024.100026","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>The present study focuses on column chromatography of ethyl acetate fractions and re fractions of <em>M.buxifolia</em> branches for potential compound isolation alongside their biological and phytochemical evaluation and TLC analysis.</p></div><div><h3>Methods</h3><p>The obtained EtOAc fractions were subjected to phytochemical (TPC, TFC), antioxidant (TRP, TAC, DPPH, MC, ABTS), antibacterial and enzyme inhibition (α-amylase, lipase and urease) assays. Fractions indicating the presence of a compound were selected for running successive columns and their factions also analyzed employing the above mentioned bioassays.</p></div><div><h3>Results</h3><p>A collective none-to-low antibacterial activity was observed. MbBE1<sub>1</sub> presented the highest antidiabetic potential (79.92%±3.9) besides moderate phytochemical and antioxidant activity. Highest phenolic content (75.85±3.6 μgGAE/mg), flavonoid content (45.39±2μgQE/mg), antioxidant capacity (99.9±3.7 μgAAE/mg), reducing power (94.82±3.9 μgAAE/mg) and radical scavenging activity (DPPH; 86.28±3%, ABTS; 98.9±2.3%, MC; 59.01±2.7%) was reported by MbBE1<sub>6</sub> making it a potential source of antioxidant agents. Column chromatography of MbBE1<sub>1</sub> produced two refractions (MbBE2<sub>1</sub> and MbBE2<sub>2</sub>) which reported moderate phytochemical and high antioxidant activity. High α-amylase and urease inhibition with low lipase inhibition was observed. MbBE2<sub>1</sub> depicted the largest ZOI against <em>S.enterica</em> (SE) (12±0.7 mm) and MbBE2<sub>2</sub> against Methicillin-resistant <em>S.aureus</em> (MRSA) (12±0.5 mm). Column chromatography of MbBE1<sub>6</sub> produced four refractions (MbBE3<sub>1</sub> – MbBE3<sub>4</sub>) with moderate phytochemical and antioxidant activity without a single dominant fraction. Antibacterial activity remained low with the average ZOI sized at 7±0.5 mm. MbBE1<sub>5</sub> reported the highest anti-urease at 95.7%±4.7 and significant lipase inhibition at 85.5%±3. MbBE1<sub>5</sub>, MbBE2<sub>2</sub> MbBE3<sub>2</sub> resulted in crystal formation later characterized as Lupeol with moderate phytochemical and antioxidant activity.</p></div><div><h3>Conclusion</h3><p>The study concludes that <em>M.buxifolia</em> is a potential source of bioactive components and can be further explored through modern strategies.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100026"},"PeriodicalIF":0.0000,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioassay guided triterpene isolation and its biological evaluation using branches extract of a significant medicinal plant; Monotheca buxifolia\",\"authors\":\"Arfah Naveed Dar , Javeria Shahzad , Joham Sarfraz Ali , Usama Sarwar , Anila Sajjad , Muhammad Zia\",\"doi\":\"10.1016/j.prenap.2024.100026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>The present study focuses on column chromatography of ethyl acetate fractions and re fractions of <em>M.buxifolia</em> branches for potential compound isolation alongside their biological and phytochemical evaluation and TLC analysis.</p></div><div><h3>Methods</h3><p>The obtained EtOAc fractions were subjected to phytochemical (TPC, TFC), antioxidant (TRP, TAC, DPPH, MC, ABTS), antibacterial and enzyme inhibition (α-amylase, lipase and urease) assays. Fractions indicating the presence of a compound were selected for running successive columns and their factions also analyzed employing the above mentioned bioassays.</p></div><div><h3>Results</h3><p>A collective none-to-low antibacterial activity was observed. MbBE1<sub>1</sub> presented the highest antidiabetic potential (79.92%±3.9) besides moderate phytochemical and antioxidant activity. Highest phenolic content (75.85±3.6 μgGAE/mg), flavonoid content (45.39±2μgQE/mg), antioxidant capacity (99.9±3.7 μgAAE/mg), reducing power (94.82±3.9 μgAAE/mg) and radical scavenging activity (DPPH; 86.28±3%, ABTS; 98.9±2.3%, MC; 59.01±2.7%) was reported by MbBE1<sub>6</sub> making it a potential source of antioxidant agents. Column chromatography of MbBE1<sub>1</sub> produced two refractions (MbBE2<sub>1</sub> and MbBE2<sub>2</sub>) which reported moderate phytochemical and high antioxidant activity. High α-amylase and urease inhibition with low lipase inhibition was observed. MbBE2<sub>1</sub> depicted the largest ZOI against <em>S.enterica</em> (SE) (12±0.7 mm) and MbBE2<sub>2</sub> against Methicillin-resistant <em>S.aureus</em> (MRSA) (12±0.5 mm). Column chromatography of MbBE1<sub>6</sub> produced four refractions (MbBE3<sub>1</sub> – MbBE3<sub>4</sub>) with moderate phytochemical and antioxidant activity without a single dominant fraction. Antibacterial activity remained low with the average ZOI sized at 7±0.5 mm. MbBE1<sub>5</sub> reported the highest anti-urease at 95.7%±4.7 and significant lipase inhibition at 85.5%±3. MbBE1<sub>5</sub>, MbBE2<sub>2</sub> MbBE3<sub>2</sub> resulted in crystal formation later characterized as Lupeol with moderate phytochemical and antioxidant activity.</p></div><div><h3>Conclusion</h3><p>The study concludes that <em>M.buxifolia</em> is a potential source of bioactive components and can be further explored through modern strategies.</p></div>\",\"PeriodicalId\":101014,\"journal\":{\"name\":\"Pharmacological Research - Natural Products\",\"volume\":\"3 \",\"pages\":\"Article 100026\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Natural Products\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950199724000144\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199724000144","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Bioassay guided triterpene isolation and its biological evaluation using branches extract of a significant medicinal plant; Monotheca buxifolia
Objective
The present study focuses on column chromatography of ethyl acetate fractions and re fractions of M.buxifolia branches for potential compound isolation alongside their biological and phytochemical evaluation and TLC analysis.
Methods
The obtained EtOAc fractions were subjected to phytochemical (TPC, TFC), antioxidant (TRP, TAC, DPPH, MC, ABTS), antibacterial and enzyme inhibition (α-amylase, lipase and urease) assays. Fractions indicating the presence of a compound were selected for running successive columns and their factions also analyzed employing the above mentioned bioassays.
Results
A collective none-to-low antibacterial activity was observed. MbBE11 presented the highest antidiabetic potential (79.92%±3.9) besides moderate phytochemical and antioxidant activity. Highest phenolic content (75.85±3.6 μgGAE/mg), flavonoid content (45.39±2μgQE/mg), antioxidant capacity (99.9±3.7 μgAAE/mg), reducing power (94.82±3.9 μgAAE/mg) and radical scavenging activity (DPPH; 86.28±3%, ABTS; 98.9±2.3%, MC; 59.01±2.7%) was reported by MbBE16 making it a potential source of antioxidant agents. Column chromatography of MbBE11 produced two refractions (MbBE21 and MbBE22) which reported moderate phytochemical and high antioxidant activity. High α-amylase and urease inhibition with low lipase inhibition was observed. MbBE21 depicted the largest ZOI against S.enterica (SE) (12±0.7 mm) and MbBE22 against Methicillin-resistant S.aureus (MRSA) (12±0.5 mm). Column chromatography of MbBE16 produced four refractions (MbBE31 – MbBE34) with moderate phytochemical and antioxidant activity without a single dominant fraction. Antibacterial activity remained low with the average ZOI sized at 7±0.5 mm. MbBE15 reported the highest anti-urease at 95.7%±4.7 and significant lipase inhibition at 85.5%±3. MbBE15, MbBE22 MbBE32 resulted in crystal formation later characterized as Lupeol with moderate phytochemical and antioxidant activity.
Conclusion
The study concludes that M.buxifolia is a potential source of bioactive components and can be further explored through modern strategies.
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