用基于肽的质谱法探测蛋白质复合物的组成、化学计量和相互作用。

4区医学 Q2 Biochemistry, Genetics and Molecular Biology

Advances in experimental medicine and biology Pub Date : 2024-01-01 DOI:10.1007/978-3-031-52193-5_4

Gianluca Degliesposti

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引用次数: 0

摘要

利用质谱分析蛋白质复合物的特征可以在不同层次上进行。最初的步骤涉及复合物的定性组成和亚基鉴定。然后，获取定量信息，如复合物或超复合物中每个亚基的化学计量比和拷贝数。基于肽的 LC-MS/MS 为蛋白质复合物的表征提供了多种方法和方案。本章主要介绍基于肽的 LC-MS/MS 在蛋白质复合物的定性、定量和结构表征方面的应用，重点是亚基鉴定、确定每个复合物中亚基的化学计量比和数量，以及交联质谱法和氢/氘交换法作为生物组装体结构研究的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Probing Protein Complexes Composition, Stoichiometry, and Interactions by Peptide-Based Mass Spectrometry.

The characterization of a protein complex by mass spectrometry can be conducted at different levels. Initial steps regard the qualitative composition of the complex and subunit identification. After that, quantitative information such as stoichiometric ratios and copy numbers for each subunit in a complex or super-complex is acquired. Peptide-based LC-MS/MS offers a wide number of methods and protocols for the characterization of protein complexes. This chapter concentrates on the applications of peptide-based LC-MS/MS for the qualitative, quantitative, and structural characterization of protein complexes focusing on subunit identification, determination of stoichiometric ratio and number of subunits per complex as well as on cross-linking mass spectrometry and hydrogen/deuterium exchange as methods for the structural investigation of the biological assemblies.

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来源期刊

Advances in experimental medicine and biology 医学-医学：研究与实验

CiteScore

5.90

自引率

0.00%

发文量

465

审稿时长

2-4 weeks

期刊介绍： Advances in Experimental Medicine and Biology provides a platform for scientific contributions in the main disciplines of the biomedicine and the life sciences. This series publishes thematic volumes on contemporary research in the areas of microbiology, immunology, neurosciences, biochemistry, biomedical engineering, genetics, physiology, and cancer research. Covering emerging topics and techniques in basic and clinical science, it brings together clinicians and researchers from various fields.