用三重标记的 13C/19F/2H 同位素模式装饰苯丙氨酸侧链。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-21 DOI:10.1007/s10858-024-00440-z

Giorgia Toscano, Julian Holzinger, Benjamin Nagl, Georg Kontaxis, Hanspeter Kählig, Robert Konrat, Roman J. Lichtenecker

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引用次数: 0

摘要

我们提出了一种经济而直接的方法，将 13C-19F 自旋系统引入苯丙氨酸的氚化芳香侧链，作为各种蛋白质核磁共振应用的报告物。该方法基于从市售同位素来源 [2-13C] 丙酮和氧化氘合成 [4-13C, 2,3,5,6-2H4] 4-氟苯丙氨酸。这种化合物很容易在含草甘膦的最小培养基中被标准大肠杆菌过表达代谢，从而在相应的目标蛋白质中产生高掺入率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns

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Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns

We present an economic and straightforward method to introduce ¹³C-¹⁹F spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-¹³C, 2,3,5,6-²H₄] 4-fluorophenylalanine from the commercially available isotope sources [2-¹³C] acetone and deuterium oxide. This compound is readily metabolized by standard Escherichia coli overexpression in a glyphosate-containing minimal medium, which results in high incorporation rates in the corresponding target proteins.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.