Neurotropin® 可改善小鼠模型中与疤痕形成相关的慢性疼痛:炎症反应的基因表达分析。

IF 2.8 3区 医学 Q2 NEUROSCIENCES
Xuan Zhou, Hiroki Iida, Yuqiang Li, Akinobu Ota, Lisheng Zhuo, Reiko Nobuhara, Yuki Terajima, Mitsuru Naiki, A Hari Reddi, Koji Kimata, Takahiro Ushida
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引用次数: 0

摘要

背景:创伤和手术后疤痕的形成涉及炎症反应,可导致慢性疼痛的发生。Neurotropin® (NTP) 是一种从接种了疫苗病毒的家兔发炎皮肤中提取的非蛋白提取物,已被广泛用于治疗慢性疼痛。然而,NTP 对疼痛性疤痕形成的体内效应尚未确定。为了研究 NTP 对炎症反应影响的分子机制,我们评估了注射 NTP 的小鼠和对照组小鼠瘢痕组织和背根神经节(DRGs)的基因表达:对照组小鼠注射生理盐水或 NTP,其他小鼠在左后爪上进行手术以诱导疼痛性疤痕的形成,然后注射生理盐水或 NTP。通过使用 von Frey 试验测量机械刺激阈值来评估后爪疼痛。在手术后四周内,小鼠后爪退缩阈值逐渐恢复到手术前的水平;经 NTP 处理的小鼠恢复时间明显缩短,约为三周,这表明 NTP 在该小鼠模型中发挥了镇痛作用。从瘢痕后爪组织中提取总 RNA,并在手术后 1 周收集 DRGs 进行芯片分析。基因组富集分析表明,一些与炎症反应相关的基因组在手术和服用 NTP 后被激活或抑制。多个基因的实时定量反转录聚合酶链反应数据与芯片分析结果一致:结论:给手术后形成疼痛疤痕的小鼠后爪注射 NTP 可减轻痛觉疼痛并减轻炎症反应。NTP 可抑制参与手术引起的炎症反应的一些基因的表达。因此,NTP 是治疗与慢性疼痛相关的疼痛疤痕的一种潜在疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neurotropin® ameliorates chronic pain associated with scar formation in a mouse model: A gene expression analysis of the inflammatory response.

Background: Scar formation after trauma and surgery involves an inflammatory response and can lead to the development of chronic pain. Neurotropin® (NTP) is a nonprotein extract of inflamed skin of rabbits inoculated with vaccinia virus. It has been widely used for the treatment of chronic pain. However, the in vivo effects of NTP on painful scar formation have not been determined. To investigate the molecular mechanisms underlying the effects of NTP on the inflammatory response, we evaluated gene expression in the scar tissues and dorsal root ganglions (DRGs) of mice administered NTP and control mice. Methods and results: Mice injected with saline or NTP were used as controls; other mice were subjected to surgery on the left hind paw to induce painful scar formation, and then injected with saline or NTP. Hind paw pain was evaluated by measuring the threshold for mechanical stimulation using the von Frey test. The paw withdrawal threshold gradually returned to pre-operative levels over 4 weeks post-operation; NTP-treated mice showed a significantly shortened recovery time of approximately 3 weeks, suggesting that NTP exerted an analgesic effect in this mouse model. Total RNA was extracted from the scarred hind paw tissues and DRGs were collected 1 week post-operation for a microarray analysis. Gene set enrichment analysis revealed that the expression of some gene sets related to inflammatory responses was activated or inhibited following surgery and NTP administration. Quantitative real-time reverse transcription-polymerase chain reaction analysis results for several genes were consistent with the microarray results. Conclusion: The administration of NTP to the hind paws of mice with painful scar formation following surgery diminished nociceptive pain and reduced the inflammatory response. NTP inhibited the expression of some genes involved in the response to surgery-induced inflammation. Therefore, NTP is a potential therapeutic option for painful scar associated with chronic pain.

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来源期刊
Molecular Pain
Molecular Pain 医学-神经科学
CiteScore
5.60
自引率
3.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Molecular Pain is a peer-reviewed, open access journal that considers manuscripts in pain research at the cellular, subcellular and molecular levels. Molecular Pain provides a forum for molecular pain scientists to communicate their research findings in a targeted manner to others in this important and growing field.
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