B M'rabet Samaali, A Loulou, A MougouHamdane, S Kallel
{"title":"Xiphinema index 和 Xiphinema italiae (Longidoridae) 获取和传播葡萄扇叶病毒 (GFLV)。","authors":"B M'rabet Samaali, A Loulou, A MougouHamdane, S Kallel","doi":"10.1017/S0022149X24000154","DOIUrl":null,"url":null,"abstract":"<p><p><i>Grapevine fanleaf virus</i> (GFLV) is one of the most severe virus diseases of grapevines, causing fanleaf degeneration that is transmitted by <i>Xiphinema index.</i> This paper aims to isolate <i>Xiphinema</i> species from Tunisian vineyard soil samples and assess their ability to acquire and transmit GFLV under natural and controlled conditions. Based on morphological and morphometric analyses, Tunisian dagger nematodes were identified as <i>X. index</i> and <i>Xiphinema italiae.</i> These results were confirmed with molecular identification tools using species-specific polymerase chain reaction primers. The total RNA of GFLV was extracted from specimens of <i>Xiphinema</i> and amplified based on real-time polymerase chain reaction using virus-specific primers. Our results showed that <i>X. index</i> could acquire and transmit the viral particles of GFLV. This nepovirus was not detected in <i>X. italiae</i>, under natural conditions; however, under controlled conditions, this nematode was able to successfully acquire and transmit the viral particles of GFLV.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Acquisition and transmission of <i>Grapevine fanleaf virus</i> (GFLV) by <i>Xiphinema index</i> and <i>Xiphinema italiae</i> (Longidoridae).\",\"authors\":\"B M'rabet Samaali, A Loulou, A MougouHamdane, S Kallel\",\"doi\":\"10.1017/S0022149X24000154\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Grapevine fanleaf virus</i> (GFLV) is one of the most severe virus diseases of grapevines, causing fanleaf degeneration that is transmitted by <i>Xiphinema index.</i> This paper aims to isolate <i>Xiphinema</i> species from Tunisian vineyard soil samples and assess their ability to acquire and transmit GFLV under natural and controlled conditions. Based on morphological and morphometric analyses, Tunisian dagger nematodes were identified as <i>X. index</i> and <i>Xiphinema italiae.</i> These results were confirmed with molecular identification tools using species-specific polymerase chain reaction primers. The total RNA of GFLV was extracted from specimens of <i>Xiphinema</i> and amplified based on real-time polymerase chain reaction using virus-specific primers. Our results showed that <i>X. index</i> could acquire and transmit the viral particles of GFLV. This nepovirus was not detected in <i>X. italiae</i>, under natural conditions; however, under controlled conditions, this nematode was able to successfully acquire and transmit the viral particles of GFLV.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-03-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1017/S0022149X24000154\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1017/S0022149X24000154","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Acquisition and transmission of Grapevine fanleaf virus (GFLV) by Xiphinema index and Xiphinema italiae (Longidoridae).
Grapevine fanleaf virus (GFLV) is one of the most severe virus diseases of grapevines, causing fanleaf degeneration that is transmitted by Xiphinema index. This paper aims to isolate Xiphinema species from Tunisian vineyard soil samples and assess their ability to acquire and transmit GFLV under natural and controlled conditions. Based on morphological and morphometric analyses, Tunisian dagger nematodes were identified as X. index and Xiphinema italiae. These results were confirmed with molecular identification tools using species-specific polymerase chain reaction primers. The total RNA of GFLV was extracted from specimens of Xiphinema and amplified based on real-time polymerase chain reaction using virus-specific primers. Our results showed that X. index could acquire and transmit the viral particles of GFLV. This nepovirus was not detected in X. italiae, under natural conditions; however, under controlled conditions, this nematode was able to successfully acquire and transmit the viral particles of GFLV.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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