蛋白水解酶作用下透明软骨匀浆中生物聚合物水解程度的比较分析

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology
T. I. Nikolaeva, D. A. Barsuk, M. V. Molchanov, D. A. Prokhorov, V. I. Emelyanenko, P. V. Shekhovtsov
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引用次数: 0

摘要

摘要 研究水解程度取决于各种蛋白水解酶的作用,这是开发结缔组织营养保健品用于生物医学的任务之一。在胰蛋白酶、糜蛋白酶、木瓜蛋白酶和含有木瓜蛋白酶的蛋白水解药物卡里巴嗪的作用下,对牛和猪气管透明软骨匀浆中的生物聚合物进行了水解。结果表明,MedFlorina 公司生产的卡里巴嗪比 Vifitech 公司生产的卡里巴嗪更有效,在温度为 60°C 和卡里巴嗪浓度为 10%时,胶原蛋白的水解程度最大。在牛的透明软骨匀浆中观察到蛋白多糖水解得更彻底,因为在核磁共振光谱中发现了糖胺聚糖水解的最终产物葡萄糖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comparative Analysis of the Degree of Hydrolysis of Biopolymers in Hyaline Cartilage Homogenates in the Presence of Proteolytic Enzymes

Comparative Analysis of the Degree of Hydrolysis of Biopolymers in Hyaline Cartilage Homogenates in the Presence of Proteolytic Enzymes

Comparative Analysis of the Degree of Hydrolysis of Biopolymers in Hyaline Cartilage Homogenates in the Presence of Proteolytic Enzymes

The study of the degree of hydrolysis depending on the action of various proteolytic enzymes is one of the tasks in the development of nutraceuticals from connective tissues for biomedicine. Hydrolysis of biopolymers in hyaline cartilage homogenates from the trachea of cattle and pigs was carried out under the action of the enzymes pancreatin, chymopsin, papain, and the proteolytic drug karipazim containing papain. It has been shown that karipazim produced by MedFlorina acted more effectively than karipazim produced by Vifitech, and the degree of collagen hydrolysis was maximal at 60°C and a concentration of karipazim of 10%. A more complete hydrolysis of proteoglycans was observed in the homogenates of hyaline cartilage of cattle, since glucose, the final product of glycosaminoglycan hydrolysis, was identified on NMR spectra.

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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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