Mina Zifar, Homayon Ahmad Panahi, Maryam Daghighi Asli, Elham Moniri, Maryam Norouzzadeh Chegini
{"title":"基于二硫化钨纳米片的双响应纳米载体的设计与开发及其对 PC-3 细胞的细胞毒性效应作为氟他胺的高效体外给药系统","authors":"Mina Zifar, Homayon Ahmad Panahi, Maryam Daghighi Asli, Elham Moniri, Maryam Norouzzadeh Chegini","doi":"10.1007/s10876-024-02588-y","DOIUrl":null,"url":null,"abstract":"<p>In this work, flutamide‐loaded tungsten disulfide nanosheets modified thermo-responsive polymer (N-vinylcaprolactam) were prepared as a dual responsive system for targeted delivery of flutamide to tumor cells. The prepared nanocarrier was characterized using field-emission scanning electron microscopy, atomic force microscopy, Zeta potential, Fourier-transform infrared spectroscopy, X-ray diffraction, and thermal gravimetric analyses. The behavior of flutamide sorption was investigated by non-linear isotherm models that the equilibrium data well fitted with the Langmuir model. The maximum sorption capacity of the drug as computed from the non-linear Langmuir model was 7.33 mg g<sup>–1</sup>. Moreover, the kinetics of the sorption procedure followed by the non-linear pseudo-2nd-order kinetic model with a higher regression coefficient (R<sup>2</sup> = 0.9965). The pH and temperature-responsive nanocarrier released minimal amounts (15.75%) of the drug at a simulated physiological medium (pH 7.4; T = 37 °C). In contrast, a fast release of the drug (77.52%) was shown in a simulated cancer medium at a high temperature after 6 h (pH 5.6; T = 45 °C). Furthermore, the released amounts of drug from the nanocarrier can be regulated by adjusting the near-infrared laser irradiation. Dual responsive nanocarrier shown targeted cytotoxicity towards PC-3 cells with milder cytotoxicity towards normal cells. The nanocarrier showed two-fold higher cytotoxicity under near-infrared laser irradiation in comparison to the nanocarrier without irradiation in the PC-3 cell lines. Enhanced antitumor efficacy by photo-thermal therapy was achieved with near-infrared laser irradiation. All these data suggest that the pH and temperature dual responsive nanocarrier is a biocompatible and efficient candidate for the specific delivery of flutamide to PC-3 cells and could be utilized as a prostate cancer specific drug delivery system.</p>","PeriodicalId":618,"journal":{"name":"Journal of Cluster Science","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Design and Development of Dual Responsive Nanocarrier Based on Tungsten Disulfide Nanosheets and Its Cytotoxic Effect on PC-3 Cells as an Efficient In Vitro Drug Delivery System for Flutamide\",\"authors\":\"Mina Zifar, Homayon Ahmad Panahi, Maryam Daghighi Asli, Elham Moniri, Maryam Norouzzadeh Chegini\",\"doi\":\"10.1007/s10876-024-02588-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In this work, flutamide‐loaded tungsten disulfide nanosheets modified thermo-responsive polymer (N-vinylcaprolactam) were prepared as a dual responsive system for targeted delivery of flutamide to tumor cells. The prepared nanocarrier was characterized using field-emission scanning electron microscopy, atomic force microscopy, Zeta potential, Fourier-transform infrared spectroscopy, X-ray diffraction, and thermal gravimetric analyses. The behavior of flutamide sorption was investigated by non-linear isotherm models that the equilibrium data well fitted with the Langmuir model. The maximum sorption capacity of the drug as computed from the non-linear Langmuir model was 7.33 mg g<sup>–1</sup>. Moreover, the kinetics of the sorption procedure followed by the non-linear pseudo-2nd-order kinetic model with a higher regression coefficient (R<sup>2</sup> = 0.9965). The pH and temperature-responsive nanocarrier released minimal amounts (15.75%) of the drug at a simulated physiological medium (pH 7.4; T = 37 °C). In contrast, a fast release of the drug (77.52%) was shown in a simulated cancer medium at a high temperature after 6 h (pH 5.6; T = 45 °C). Furthermore, the released amounts of drug from the nanocarrier can be regulated by adjusting the near-infrared laser irradiation. Dual responsive nanocarrier shown targeted cytotoxicity towards PC-3 cells with milder cytotoxicity towards normal cells. The nanocarrier showed two-fold higher cytotoxicity under near-infrared laser irradiation in comparison to the nanocarrier without irradiation in the PC-3 cell lines. Enhanced antitumor efficacy by photo-thermal therapy was achieved with near-infrared laser irradiation. All these data suggest that the pH and temperature dual responsive nanocarrier is a biocompatible and efficient candidate for the specific delivery of flutamide to PC-3 cells and could be utilized as a prostate cancer specific drug delivery system.</p>\",\"PeriodicalId\":618,\"journal\":{\"name\":\"Journal of Cluster Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-03-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cluster Science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s10876-024-02588-y\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cluster Science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10876-024-02588-y","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Design and Development of Dual Responsive Nanocarrier Based on Tungsten Disulfide Nanosheets and Its Cytotoxic Effect on PC-3 Cells as an Efficient In Vitro Drug Delivery System for Flutamide
In this work, flutamide‐loaded tungsten disulfide nanosheets modified thermo-responsive polymer (N-vinylcaprolactam) were prepared as a dual responsive system for targeted delivery of flutamide to tumor cells. The prepared nanocarrier was characterized using field-emission scanning electron microscopy, atomic force microscopy, Zeta potential, Fourier-transform infrared spectroscopy, X-ray diffraction, and thermal gravimetric analyses. The behavior of flutamide sorption was investigated by non-linear isotherm models that the equilibrium data well fitted with the Langmuir model. The maximum sorption capacity of the drug as computed from the non-linear Langmuir model was 7.33 mg g–1. Moreover, the kinetics of the sorption procedure followed by the non-linear pseudo-2nd-order kinetic model with a higher regression coefficient (R2 = 0.9965). The pH and temperature-responsive nanocarrier released minimal amounts (15.75%) of the drug at a simulated physiological medium (pH 7.4; T = 37 °C). In contrast, a fast release of the drug (77.52%) was shown in a simulated cancer medium at a high temperature after 6 h (pH 5.6; T = 45 °C). Furthermore, the released amounts of drug from the nanocarrier can be regulated by adjusting the near-infrared laser irradiation. Dual responsive nanocarrier shown targeted cytotoxicity towards PC-3 cells with milder cytotoxicity towards normal cells. The nanocarrier showed two-fold higher cytotoxicity under near-infrared laser irradiation in comparison to the nanocarrier without irradiation in the PC-3 cell lines. Enhanced antitumor efficacy by photo-thermal therapy was achieved with near-infrared laser irradiation. All these data suggest that the pH and temperature dual responsive nanocarrier is a biocompatible and efficient candidate for the specific delivery of flutamide to PC-3 cells and could be utilized as a prostate cancer specific drug delivery system.
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