{"title":"基于连续荧光原位杂交分析的蒿属植物(菊科:防风草属)10 个卫星重复序列的分化:物种鉴定和进化的证据","authors":"Yanze He, Jun He, Yong Zhao, Shuangshuang Zhang, Xinyu Rao, Haibin Wang, Zhenxing Wang, Aiping Song, Jiafu Jiang, Sumei Chen, Fadi Chen","doi":"10.1007/s10577-024-09749-9","DOIUrl":null,"url":null,"abstract":"<p><i>Artemisia</i> is a large genus encompassing about 400 diverse species, many of which have considerable medicinal and ecological value. However, complex morphological information and variation in ploidy level and nuclear DNA content have presented challenges for evolution studies of this genus. Consequently, taxonomic inconsistencies within the genus persist, hindering the utilization of such large plant resources. Researchers have utilized satellite DNAs to aid in chromosome identification, species classification, and evolutionary studies due to their significant sequence and copy number variation between species and close relatives. In the present study, the RepeatExplorer2 pipeline was utilized to identify 10 satellite DNAs from three species (<i>Artemisia annua</i>, <i>Artemisia vulgaris</i>, <i>Artemisia viridisquama</i>), and fluorescence in situ hybridization confirmed their distribution on chromosomes in 24 species, including 19 <i>Artemisia</i> species with 5 outgroup species from <i>Ajania</i> and <i>Chrysanthemum</i>. Signals of satellite DNAs exhibited substantial differences between species. We obtained one genus-specific satellite from the sequences. Additionally, molecular cytogenetic maps were constructed for <i>Artemisia vulgaris</i>, <i>Artemisia leucophylla</i>, and <i>Artemisia viridisquama</i>. One species (<i>Artemisia verbenacea</i>) showed a FISH distribution pattern suggestive of an allotriploid origin. Heteromorphic FISH signals between homologous chromosomes in <i>Artemisia</i> plants were observed at a high level. Additionally, the relative relationships between species were discussed by comparing ideograms. The results of the present study provide new insights into the accurate identification and taxonomy of the <i>Artemisia</i> genus using molecular cytological methods.</p>","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"16 1","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Divergence of 10 satellite repeats in Artemisia (Asteraceae: Anthemideae) based on sequential fluorescence in situ hybridization analysis: evidence for species identification and evolution\",\"authors\":\"Yanze He, Jun He, Yong Zhao, Shuangshuang Zhang, Xinyu Rao, Haibin Wang, Zhenxing Wang, Aiping Song, Jiafu Jiang, Sumei Chen, Fadi Chen\",\"doi\":\"10.1007/s10577-024-09749-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Artemisia</i> is a large genus encompassing about 400 diverse species, many of which have considerable medicinal and ecological value. However, complex morphological information and variation in ploidy level and nuclear DNA content have presented challenges for evolution studies of this genus. Consequently, taxonomic inconsistencies within the genus persist, hindering the utilization of such large plant resources. Researchers have utilized satellite DNAs to aid in chromosome identification, species classification, and evolutionary studies due to their significant sequence and copy number variation between species and close relatives. In the present study, the RepeatExplorer2 pipeline was utilized to identify 10 satellite DNAs from three species (<i>Artemisia annua</i>, <i>Artemisia vulgaris</i>, <i>Artemisia viridisquama</i>), and fluorescence in situ hybridization confirmed their distribution on chromosomes in 24 species, including 19 <i>Artemisia</i> species with 5 outgroup species from <i>Ajania</i> and <i>Chrysanthemum</i>. Signals of satellite DNAs exhibited substantial differences between species. We obtained one genus-specific satellite from the sequences. Additionally, molecular cytogenetic maps were constructed for <i>Artemisia vulgaris</i>, <i>Artemisia leucophylla</i>, and <i>Artemisia viridisquama</i>. One species (<i>Artemisia verbenacea</i>) showed a FISH distribution pattern suggestive of an allotriploid origin. Heteromorphic FISH signals between homologous chromosomes in <i>Artemisia</i> plants were observed at a high level. Additionally, the relative relationships between species were discussed by comparing ideograms. The results of the present study provide new insights into the accurate identification and taxonomy of the <i>Artemisia</i> genus using molecular cytological methods.</p>\",\"PeriodicalId\":50698,\"journal\":{\"name\":\"Chromosome Research\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-03-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromosome Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10577-024-09749-9\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10577-024-09749-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
蒿属是一个庞大的属,包括约 400 个不同的物种,其中许多物种具有相当高的药用和生态价值。然而,复杂的形态信息以及倍性水平和核 DNA 含量的差异给该属的进化研究带来了挑战。因此,属内分类不一致的情况依然存在,阻碍了对如此庞大的植物资源的利用。由于卫星 DNA 在物种和近缘种之间存在显著的序列和拷贝数差异,研究人员利用卫星 DNA 协助进行染色体鉴定、物种分类和进化研究。在本研究中,研究人员利用 RepeatExplorer2 管道从 3 个物种(黄花蒿、寻常蒿和蒿属植物)中鉴定出了 10 个卫星 DNA,并通过荧光原位杂交确认了它们在 24 个物种染色体上的分布,其中包括 19 个蒿属植物物种以及 5 个来自蒿属植物和菊属植物的外群物种。卫星 DNA 的信号在不同物种之间存在很大差异。我们从这些序列中获得了一个种属特异性卫星。此外,我们还构建了寻常蒿(Artemisia vulgaris)、白花蒿(Artemisia leucophylla)和黄花蒿(Artemisia viridisquama)的分子细胞遗传图谱。其中一个物种(Artemisia verbenacea)的 FISH 分布模式表明其起源于异源三倍体。在蒿属植物的同源染色体之间观察到了高水平的异形 FISH 信号。此外,还通过比较表意图讨论了物种之间的相对关系。本研究的结果为利用分子细胞学方法准确鉴定和分类蒿属提供了新的见解。
Divergence of 10 satellite repeats in Artemisia (Asteraceae: Anthemideae) based on sequential fluorescence in situ hybridization analysis: evidence for species identification and evolution
Artemisia is a large genus encompassing about 400 diverse species, many of which have considerable medicinal and ecological value. However, complex morphological information and variation in ploidy level and nuclear DNA content have presented challenges for evolution studies of this genus. Consequently, taxonomic inconsistencies within the genus persist, hindering the utilization of such large plant resources. Researchers have utilized satellite DNAs to aid in chromosome identification, species classification, and evolutionary studies due to their significant sequence and copy number variation between species and close relatives. In the present study, the RepeatExplorer2 pipeline was utilized to identify 10 satellite DNAs from three species (Artemisia annua, Artemisia vulgaris, Artemisia viridisquama), and fluorescence in situ hybridization confirmed their distribution on chromosomes in 24 species, including 19 Artemisia species with 5 outgroup species from Ajania and Chrysanthemum. Signals of satellite DNAs exhibited substantial differences between species. We obtained one genus-specific satellite from the sequences. Additionally, molecular cytogenetic maps were constructed for Artemisia vulgaris, Artemisia leucophylla, and Artemisia viridisquama. One species (Artemisia verbenacea) showed a FISH distribution pattern suggestive of an allotriploid origin. Heteromorphic FISH signals between homologous chromosomes in Artemisia plants were observed at a high level. Additionally, the relative relationships between species were discussed by comparing ideograms. The results of the present study provide new insights into the accurate identification and taxonomy of the Artemisia genus using molecular cytological methods.
期刊介绍:
Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to:
· Chromosomes and their linkage to diseases;
· Chromosome organization within the nucleus;
· Chromatin biology (transcription, non-coding RNA, etc);
· Chromosome structure, function and mechanics;
· Chromosome and DNA repair;
· Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting,
dosage compensation, sex determination, chromosome remodeling);
· Architectural/epigenomic organization of the genome;
· Functional annotation of the genome;
· Functional and comparative genomics in plants and animals;
· Karyology studies that help resolve difficult taxonomic problems or that provide
clues to fundamental mechanisms of genome and karyotype evolution in plants and animals;
· Mitosis and Meiosis;
· Cancer cytogenomics.