I. N. Kogarko, V. V. Petushkova, B. S. Kogarko, E. A. Pryakhin, E. A. Neyfakh, O. V. Ktitorova, S. S. Andreev, I. I. Ganeev, N. S. Kuzmina, E. I. Selivanova, I. I. Pelevina
{"title":"从机体层面研究电离辐射对辐射诱导的小鼠血液系统细胞变化的影响","authors":"I. N. Kogarko, V. V. Petushkova, B. S. Kogarko, E. A. Pryakhin, E. A. Neyfakh, O. V. Ktitorova, S. S. Andreev, I. I. Ganeev, N. S. Kuzmina, E. I. Selivanova, I. I. Pelevina","doi":"10.1134/S1990750823600292","DOIUrl":null,"url":null,"abstract":"<p>The task of studying radiation “bystander effects” at the organism level was set in the work. Irradiated and nonirradiated mice that were kept together were used in the experiment. Mice were irradiated at a dose of 3 Gy in a research radiobiological gamma installation with the sources of <sup>137</sup>Cs. The number of leukocytes and relative number of lymphocytes in the peripheral blood of mice were estimated 3, 7, 14, 30, 60, and 90 days after the beginning of the experiment. In nonirradiated “bystander” mice kept with irradiated mice both in the cages without a partition and with a partition, there is a possible trend towards a decrease in the number of leukocytes as compared with indices in the biocontrol. During the analysis of relative number of lymphocytes, a trend towards a decrease in the index in “bystander” mice was demonstrated both when keeping without a partition and with a partition. In nonirradiated “bystander” animals when keeping with irradiated animals in the cage with a partition, a statistically significant decrease in the relative number of lymphocytes in the peripheral blood was registered on days 3 (<i>t</i> = 2.13; <i>p</i> = 0.047), 30 (<i>t</i> = 2.94; <i>p</i> = 0.01), and 90 (<i>t</i> = 3.07; <i>p</i> = 0.01) after irradiation as well as when keeping in the same cage with irradiated mice without a partition on day 60 (<i>t</i> = 2.24; <i>p</i> = 0.038) after the beginning of the experiment. A “bystander effect” in nonirradiated animals is one of possible explanations for the detected changes. In irradiated animals that were kept in the same cage with a partition together with nonirradiated animals, a statistically significant increase in the relative number of lymphocytes was registered on days 3 (<i>t</i> = 2.6; <i>p</i> = 0.02), 14 (<i>t</i> = 2.61; <i>p</i> = 0.018), and 60 (<i>t</i> = 2.38; <i>p</i> = 0.03) (“rescue effect”). Based on data obtained in the present experiment, it is assumed that the radiation “bystander effect” can have the opposite nature; that is, nonirradiated organisms are able to reduce the radiation effects in irradiated individuals (“rescue effect”).</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"17 3","pages":"145 - 151"},"PeriodicalIF":0.6000,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Studying the Effect of Ionizing Radiation on Radiation-Induced Changes in the Cells of the Blood System in Mice at the Organism Level\",\"authors\":\"I. N. Kogarko, V. V. Petushkova, B. S. Kogarko, E. A. Pryakhin, E. A. Neyfakh, O. V. Ktitorova, S. S. Andreev, I. I. Ganeev, N. S. Kuzmina, E. I. Selivanova, I. I. Pelevina\",\"doi\":\"10.1134/S1990750823600292\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The task of studying radiation “bystander effects” at the organism level was set in the work. Irradiated and nonirradiated mice that were kept together were used in the experiment. Mice were irradiated at a dose of 3 Gy in a research radiobiological gamma installation with the sources of <sup>137</sup>Cs. The number of leukocytes and relative number of lymphocytes in the peripheral blood of mice were estimated 3, 7, 14, 30, 60, and 90 days after the beginning of the experiment. In nonirradiated “bystander” mice kept with irradiated mice both in the cages without a partition and with a partition, there is a possible trend towards a decrease in the number of leukocytes as compared with indices in the biocontrol. During the analysis of relative number of lymphocytes, a trend towards a decrease in the index in “bystander” mice was demonstrated both when keeping without a partition and with a partition. In nonirradiated “bystander” animals when keeping with irradiated animals in the cage with a partition, a statistically significant decrease in the relative number of lymphocytes in the peripheral blood was registered on days 3 (<i>t</i> = 2.13; <i>p</i> = 0.047), 30 (<i>t</i> = 2.94; <i>p</i> = 0.01), and 90 (<i>t</i> = 3.07; <i>p</i> = 0.01) after irradiation as well as when keeping in the same cage with irradiated mice without a partition on day 60 (<i>t</i> = 2.24; <i>p</i> = 0.038) after the beginning of the experiment. A “bystander effect” in nonirradiated animals is one of possible explanations for the detected changes. In irradiated animals that were kept in the same cage with a partition together with nonirradiated animals, a statistically significant increase in the relative number of lymphocytes was registered on days 3 (<i>t</i> = 2.6; <i>p</i> = 0.02), 14 (<i>t</i> = 2.61; <i>p</i> = 0.018), and 60 (<i>t</i> = 2.38; <i>p</i> = 0.03) (“rescue effect”). Based on data obtained in the present experiment, it is assumed that the radiation “bystander effect” can have the opposite nature; that is, nonirradiated organisms are able to reduce the radiation effects in irradiated individuals (“rescue effect”).</p>\",\"PeriodicalId\":485,\"journal\":{\"name\":\"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry\",\"volume\":\"17 3\",\"pages\":\"145 - 151\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-03-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1990750823600292\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1134/S1990750823600292","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Studying the Effect of Ionizing Radiation on Radiation-Induced Changes in the Cells of the Blood System in Mice at the Organism Level
The task of studying radiation “bystander effects” at the organism level was set in the work. Irradiated and nonirradiated mice that were kept together were used in the experiment. Mice were irradiated at a dose of 3 Gy in a research radiobiological gamma installation with the sources of 137Cs. The number of leukocytes and relative number of lymphocytes in the peripheral blood of mice were estimated 3, 7, 14, 30, 60, and 90 days after the beginning of the experiment. In nonirradiated “bystander” mice kept with irradiated mice both in the cages without a partition and with a partition, there is a possible trend towards a decrease in the number of leukocytes as compared with indices in the biocontrol. During the analysis of relative number of lymphocytes, a trend towards a decrease in the index in “bystander” mice was demonstrated both when keeping without a partition and with a partition. In nonirradiated “bystander” animals when keeping with irradiated animals in the cage with a partition, a statistically significant decrease in the relative number of lymphocytes in the peripheral blood was registered on days 3 (t = 2.13; p = 0.047), 30 (t = 2.94; p = 0.01), and 90 (t = 3.07; p = 0.01) after irradiation as well as when keeping in the same cage with irradiated mice without a partition on day 60 (t = 2.24; p = 0.038) after the beginning of the experiment. A “bystander effect” in nonirradiated animals is one of possible explanations for the detected changes. In irradiated animals that were kept in the same cage with a partition together with nonirradiated animals, a statistically significant increase in the relative number of lymphocytes was registered on days 3 (t = 2.6; p = 0.02), 14 (t = 2.61; p = 0.018), and 60 (t = 2.38; p = 0.03) (“rescue effect”). Based on data obtained in the present experiment, it is assumed that the radiation “bystander effect” can have the opposite nature; that is, nonirradiated organisms are able to reduce the radiation effects in irradiated individuals (“rescue effect”).
期刊介绍：
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.
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