{"title":"质量可靠的 R-phycoerythrin 提取自 Portieria hornemannii，具有有效的抗氧化、抗菌和抗癌活性","authors":"Saranya Karuppannan , Mani Sivakumar , Balasubramani Govindasamy , Santhosh Chinnaraj , Viji Maluventhan , Maruthupandian Arumugam","doi":"10.1016/j.bea.2024.100116","DOIUrl":null,"url":null,"abstract":"<div><p>The phycobiliprotein is a light-harvesting fluorescent probe; Phycoerythrin is a pigment used in many industrial, and pharmaceutical applications; including fluorescence resonance energy transfer and flow cytometry. The main objective of the current study was purifying the phycoerythrin in <em>Portieria hornemannii</em>. The phycoerythrin pigment was dialyzed commonly using Q sepharose, but it is high cost. However, the alternative method is DEAE-Sephadex is cost-effective, high- yield, pure compound, and has high activity performance. Then the R-Phycoerythrin (R-PE) compound was extracted in 0.02 M of phosphate buffer (pH–7.2). R-PE investigated the FT-IR, HPLC, SDS-PAGE and including physico-chemical properties. Then the purified R-PE compound was evaluated by an antibacterial, antioxidant, and anticancer activity. The R-Phycoerythrin was showing a maximum inhibition effect of high concentration (IC<sub>50</sub>value 77.98 µg/ml) against the colon cancer HT-29 cell line.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100116"},"PeriodicalIF":0.0000,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000057/pdfft?md5=002935a3085e505b6e3bda709475f7f0&pid=1-s2.0-S2667099224000057-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Reliable quality of R-phycoerythrin derived from Portieria hornemannii for effective antioxidant, antibacterial, and anticancer activity\",\"authors\":\"Saranya Karuppannan , Mani Sivakumar , Balasubramani Govindasamy , Santhosh Chinnaraj , Viji Maluventhan , Maruthupandian Arumugam\",\"doi\":\"10.1016/j.bea.2024.100116\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The phycobiliprotein is a light-harvesting fluorescent probe; Phycoerythrin is a pigment used in many industrial, and pharmaceutical applications; including fluorescence resonance energy transfer and flow cytometry. The main objective of the current study was purifying the phycoerythrin in <em>Portieria hornemannii</em>. The phycoerythrin pigment was dialyzed commonly using Q sepharose, but it is high cost. However, the alternative method is DEAE-Sephadex is cost-effective, high- yield, pure compound, and has high activity performance. Then the R-Phycoerythrin (R-PE) compound was extracted in 0.02 M of phosphate buffer (pH–7.2). R-PE investigated the FT-IR, HPLC, SDS-PAGE and including physico-chemical properties. Then the purified R-PE compound was evaluated by an antibacterial, antioxidant, and anticancer activity. The R-Phycoerythrin was showing a maximum inhibition effect of high concentration (IC<sub>50</sub>value 77.98 µg/ml) against the colon cancer HT-29 cell line.</p></div>\",\"PeriodicalId\":72384,\"journal\":{\"name\":\"Biomedical engineering advances\",\"volume\":\"7 \",\"pages\":\"Article 100116\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-02-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667099224000057/pdfft?md5=002935a3085e505b6e3bda709475f7f0&pid=1-s2.0-S2667099224000057-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical engineering advances\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667099224000057\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical engineering advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667099224000057","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Reliable quality of R-phycoerythrin derived from Portieria hornemannii for effective antioxidant, antibacterial, and anticancer activity
The phycobiliprotein is a light-harvesting fluorescent probe; Phycoerythrin is a pigment used in many industrial, and pharmaceutical applications; including fluorescence resonance energy transfer and flow cytometry. The main objective of the current study was purifying the phycoerythrin in Portieria hornemannii. The phycoerythrin pigment was dialyzed commonly using Q sepharose, but it is high cost. However, the alternative method is DEAE-Sephadex is cost-effective, high- yield, pure compound, and has high activity performance. Then the R-Phycoerythrin (R-PE) compound was extracted in 0.02 M of phosphate buffer (pH–7.2). R-PE investigated the FT-IR, HPLC, SDS-PAGE and including physico-chemical properties. Then the purified R-PE compound was evaluated by an antibacterial, antioxidant, and anticancer activity. The R-Phycoerythrin was showing a maximum inhibition effect of high concentration (IC50value 77.98 µg/ml) against the colon cancer HT-29 cell line.
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