两种不同的 SNARE 复合物介导囊泡与质膜的融合,以确保 Fusarium oxysporum f. sp. cubense 的有效发育和致病。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Zhenyu Fang, Qiwen Zhao, Shiyu Yang, Yan Cai, Wenqin Fang, Yakubu Saddeeq Abubakar, Ying Lin, Yingzi Yun, Wenhui Zheng
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引用次数: 0

摘要

SNARE(可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体)可促进囊泡与目标膜的对接和融合,在囊泡贩运和外吞过程中发挥着至关重要的作用。然而,人们对与质膜(PM)相关的SNAREs在植物病原体发育和致病过程中的空间组装和作用尚不清楚。在本研究中,我们分析了与质膜相关的 SNARE 复合物在香蕉镰刀菌枯萎病真菌 Fusarium oxysporum f. sp. cubense tropical race 4(FocTR4)中的作用和分子机制。我们的研究结果表明,FocSso1 对真菌的生长、分生孢子、宿主穿透和定殖非常重要。从机制上讲,FocSso1 通过介导囊泡与 PM 和头状花序顶端的对接和融合来调节蛋白质的分泌。有趣的是,据观察,FocSso1-FocSec9-FocSnc1 复合物不仅在真菌原生质部聚集,而且还在不断生长的菌头顶端聚集,从而促进了外泌。FocSso2 是 FocSso1 的旁系亲属,也被发现与 FocSec9 和 FocSnc1 形成三元 SNARE 复合物,但它主要定位于老菌丝的 PM。对该蛋白的功能分析表明,它对真菌的生长是不可或缺的,但对宿主的穿透和定殖却是必要的。其他亚基 FocSec9 和 FocSnc1 则参与真菌的生长发育并促进宿主的穿透。此外,FocSso1 和 FocSnc1 在功能上相互依赖,因为 FocSso1 的缺失会导致 FocSnc1 在液泡中的错误分类和降解,反之亦然。总之,这项研究深入揭示了两种空间和功能上不同的 PM SNARE 复合物的形成及其参与囊泡外排的过程,从而调控 FocTR4 的发育和致病性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense.

Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense.

SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) facilitate docking and fusion of vesicles with their target membranes, playing a crucial role in vesicle trafficking and exocytosis. However, the spatial assembly and roles of plasma membrane (PM)-associated SNAREs in phytopathogen development and pathogenicity are not clearly understood. In this study, we analysed the roles and molecular mechanisms of PM-associated SNARE complexes in the banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Our findings demonstrate that FocSso1 is important for the fungal growth, conidiation, host penetration and colonization. Mechanistically, FocSso1 regulates protein secretion by mediating vesicle docking and fusion with the PM and hyphal apex. Interestingly, a FocSso1-FocSec9-FocSnc1 complex was observed to assemble not only at the fungal PM but also on the growing hyphal apex, facilitating exocytosis. FocSso2, a paralogue of FocSso1, was also found to form a ternary SNARE complex with FocSec9 and FocSnc1, but it mainly localizes to the PM in old hyphae. The functional analysis of this protein demonstrated that it is dispensable for the fungal growth but necessary for host penetration and colonization. The other subunits, FocSec9 and FocSnc1, are involved in the fungal development and facilitate host penetration. Furthermore, FocSso1 and FocSnc1 are functionally interdependent, as loss of FocSso1 leads to mis-sorting and degradation of FocSnc1 in the vacuole and vice versa. Overall, this study provides insight into the formation of two spatially and functionally distinct PM SNARE complexes and their involvement in vesicle exocytosis to regulate development and pathogenicity of FocTR4.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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