Camilo Castellar-Mendoza, María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero, Carolina Coronel-Ruiz, Sigrid Camacho-Ortega, Lilia J Bernal-Cepeda, Lady López-Ibarra, Jhann A Arturo, Félix G Delgado, Hernando Gutierrez-Barbosa, Sonia Bohorquez-Avila, Johanna Madroñero, Zayda L Corredor-Rozo, Sandra J Perdomo-Lara, Angela Fonseca-Benitez, Eliana Calvo
{"title":"开发和优化用于检测人体样本中 SARS-CoV-2 的多重实时 RT-PCR 技术","authors":"Camilo Castellar-Mendoza, María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero, Carolina Coronel-Ruiz, Sigrid Camacho-Ortega, Lilia J Bernal-Cepeda, Lady López-Ibarra, Jhann A Arturo, Félix G Delgado, Hernando Gutierrez-Barbosa, Sonia Bohorquez-Avila, Johanna Madroñero, Zayda L Corredor-Rozo, Sandra J Perdomo-Lara, Angela Fonseca-Benitez, Eliana Calvo","doi":"10.1155/2024/4894004","DOIUrl":null,"url":null,"abstract":"<p><p>PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10948217/pdf/","citationCount":"0","resultStr":"{\"title\":\"Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples.\",\"authors\":\"Camilo Castellar-Mendoza, María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero, Carolina Coronel-Ruiz, Sigrid Camacho-Ortega, Lilia J Bernal-Cepeda, Lady López-Ibarra, Jhann A Arturo, Félix G Delgado, Hernando Gutierrez-Barbosa, Sonia Bohorquez-Avila, Johanna Madroñero, Zayda L Corredor-Rozo, Sandra J Perdomo-Lara, Angela Fonseca-Benitez, Eliana Calvo\",\"doi\":\"10.1155/2024/4894004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately.</p>\",\"PeriodicalId\":14098,\"journal\":{\"name\":\"International Journal of Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-03-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10948217/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/4894004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/4894004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples.
PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately.
期刊介绍：
International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
