细胞器的不对称定位反映了小鼠肌母细胞的细胞手性。

IF 6.6 3区 医学 Q1 ENGINEERING, BIOMEDICAL
APL Bioengineering Pub Date : 2024-03-14 eCollection Date: 2024-03-01 DOI:10.1063/5.0189401
Zeina Hachem, Courtney Hadrian, Lina Aldbaisi, Muslim Alkaabi, Leo Q Wan, Jie Fan
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引用次数: 0

摘要

细胞手性对生物组织的手性形态发生至关重要,但其潜在机制仍不清楚。众所周知,细胞器沿细胞体的多个轴(即顶端-基底、前端-后端和左-右)极化可指导细胞的定向、旋转和迁移等行为。在这些轴向中,左右偏向在决定这些行为的手性方向性方面具有重要影响。正常情况下,小鼠成肌细胞(C2C12)在环形微图案上表现出强烈的逆时针手性,而在 Latrunculin A 处理下则表现出顺时针主导手性。为了研究多细胞手性与单细胞细胞器定位之间的关系,我们通过微图案技术、荧光显微镜和成像分析,研究了单细胞在不同细胞手性下细胞器的左右定位。我们发现,在 "T "形微图案上,C2C12细胞呈三角形,其细胞核-中心体轴指向 "T "的右上方。其他一些细胞器,包括高尔基体、溶酶体、肌动蛋白丝和微管,都显示出偏爱极化在轴的一侧,这表明左右不对称细胞器定位的普遍性。有趣的是,当使用 Latrunculin A 扭转细胞手性时,细胞器也相应地扭转了其左右定位偏向,这与前缘始终偏向新陈代谢和收缩特性有关。细胞器定位的这种左右不对称可能有助于预测细胞迁移方向,并可作为生物模型中识别细胞手性的潜在标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Asymmetrical positioning of cell organelles reflects the cell chirality of mouse myoblast cells.

Cell chirality is crucial for the chiral morphogenesis of biological tissues, yet its underlying mechanism remains unclear. Cell organelle polarization along multiple axes in a cell body, namely, apical-basal, front-rear, and left-right, is known to direct cell behavior such as orientation, rotation, and migration. Among these axes, the left-right bias holds significant sway in determining the chiral directionality of these behaviors. Normally, mouse myoblast (C2C12) cells exhibit a strong counterclockwise chirality on a ring-shaped micropattern, whereas they display a clockwise dominant chirality under Latrunculin A treatment. To investigate the relationship between multicellular chirality and organelle positioning in single cells, we studied the left-right positioning of cell organelles under distinct cell chirality in single cells via micropatterning technique, fluorescent microscopy, and imaging analysis. We found that on a "T"-shaped micropattern, a C2C12 cell adopts a triangular shape, with its nucleus-centrosome axis pointing toward the top-right direction of the "T." Several other organelles, including the Golgi apparatus, lysosomes, actin filaments, and microtubules, showed a preference to polarize on one side of the axis, indicating the universality of the left-right asymmetrical organelle positioning. Interestingly, upon reversing cell chirality with Latrunculin A, the organelles correspondingly reversed their left-right positioning bias, as suggested by the consistently biased metabolism and contractile properties at the leading edge. This left-right asymmetry in organelle positioning may help predict cell migration direction and serve as a potential marker for identifying cell chirality in biological models.

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来源期刊
APL Bioengineering
APL Bioengineering ENGINEERING, BIOMEDICAL-
CiteScore
9.30
自引率
6.70%
发文量
39
审稿时长
19 weeks
期刊介绍: APL Bioengineering is devoted to research at the intersection of biology, physics, and engineering. The journal publishes high-impact manuscripts specific to the understanding and advancement of physics and engineering of biological systems. APL Bioengineering is the new home for the bioengineering and biomedical research communities. APL Bioengineering publishes original research articles, reviews, and perspectives. Topical coverage includes: -Biofabrication and Bioprinting -Biomedical Materials, Sensors, and Imaging -Engineered Living Systems -Cell and Tissue Engineering -Regenerative Medicine -Molecular, Cell, and Tissue Biomechanics -Systems Biology and Computational Biology
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