Uwe Wessels, Florian Neff, Julia Fakhiri, Klaus Mayer, Ulrich Brinkmann, Kay Stubenrauch
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引用次数: 0
摘要
目的：开发一种检测抗腺体相关病毒 2 (AAV2) 总抗体的检测方法，且囊壳材料消耗量低。方法：开发一种免疫复合物（IC）检测方法。该方法基于在溶液中形成 IC，然后使用抗 AAV2 抗体进行捕获和使用抗研究物种 IgG 的抗体进行检测。结果：在猕猴和人类血清样本（包括基于 AAV2 疗法的临床前研究样本）中证明了 IC 检测法检测已有的和治疗后出现的抗 AAV2 抗体的可行性。结论所介绍的 IC 检测法是一种易于操作的全抗 AAV2 抗体检测法，只需少量未标记的囊壳材料，并提供内在特异性控制。
Novel assay format for total anti-adeno-associated virus antibody detection with low capsid consumption and built-in specificity control.
Aim: To develop an assay format for detection of total anti-adeno-associated virus 2 (AAV2) antibodies with low capsid material consumption. Methods: An immune complex (IC) assay format was developed. The format is based on the formation of ICs in solution and their subsequent detection using an anti-AAV2 antibody for capture and an antibody against the study species IgG for detection. Results: The feasibility of the IC assay for detection of preexisting and treatment-emergent anti-AAV2 antibodies was demonstrated in cynomolgus monkey and human serum samples, including samples from a preclinical study with AAV2-based therapies. Conclusion: The presented IC assay is an easy-to-perform total anti-AAV2 antibody assay that requires a small amount of unlabeled capsid material and provides an intrinsic specificity control.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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