利用 LC-MS/MS 测定毛细管血浆微量样本中的美罗培南。

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-01-01 Epub Date: 2024-03-18 DOI:10.4155/bio-2023-0268

Maria Amélia de Castilhos Busato, Amanda Pacheco Bondan, Marcos Frank Bastiani, Lilian Feltraco Lizot, Roberta Zilles Hahn, Marina Venzon Antunes, Rafael Linden

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引用次数: 0

摘要

背景：测量美罗培南的血浆浓度可用于给药方案的个体化。本研究旨在开发并验证一种 LC-MS/MS 检测方法，用于定量检测毛细管血浆微量样本中的美罗培南。检测方法用乙腈沉淀蛋白制备样品，然后用二氯甲烷净化。对 12 份静脉和毛细管血浆配对样本进行了验证和应用。结果表明该方法在 0.5-50 μg/ml 范围内线性良好。基质效应极小。测定间和测定内准确度分别为 3.8-7.9%和 2.7-5.5%，准确度为 91.7-100.6%。毛细血管和静脉血浆中的浓度高度相关。结论毛细管血浆微量样本中美罗培南的定量检测方法得到了充分验证，具有临床应用潜力。

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Determination of meropenem in capillary plasma microsamples using LC-MS/MS.

Background: The measurement of meropenem plasma concentrations is employed for dosing regimen individualization. The aim of this study was to develop and validate a LC-MS/MS assay for quantification of meropenem in capillary plasma microsamples. Methods: Samples were prepared by protein precipitation with acetonitrile, followed by clean-up with dichloromethane. The method was validated and applied to 12 paired samples of venous and capillary plasma. Results: The method was linear in the range of 0.5-50 μg/ml. Matrix effects were minimal. Inter- and intra-assay were 3.8-7.9% and 2.7-5.5%, respectively, while accuracy was 91.7-100.6%. Concentrations in capillary and venous plasma were highly correlated. Conclusion: An assay for the quantification of meropenem in capillary plasma microsamples was fully validated, showing potential for clinical application.

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.