CRISPR 激活筛选：技术与应用导航。

IF 14.3 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Trends in biotechnology Pub Date : 2024-08-01 Epub Date: 2024-03-15 DOI:10.1016/j.tibtech.2024.02.007

Teleri Clark, Matthew A Waller, Lipin Loo, Cesar L Moreno, Christopher E Denes, G Gregory Neely

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引用次数: 0

摘要

聚类规则间隔短回文重复序列（CRISPR）激活（CRISPRa）已成为分子生物学工具包中不可或缺的一部分。CRISPRa 基因筛选是一种令人兴奋的高通量方法，可用于鉴定上调足以引起特定表型的基因。激活机制正在不断发展，以实现更强、更稳健和更一致的激活。在这篇综述中，我们简明扼要地介绍了现有 CRISPRa 架构的技术概况，并全面总结了集合 CRISPRa 筛选。此外，我们还讨论了 CRISPRa 在广泛研究领域中的当代应用，旨在展示 CRISPRa 筛选令人兴奋的新兴应用。

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CRISPR activation screens: navigating technologies and applications.

Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.

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来源期刊

Trends in biotechnology 工程技术-生物工程与应用微生物

CiteScore

28.60

自引率

1.20%

发文量

198

审稿时长

1 months

期刊介绍： Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).