多焦点序列分析揭示了与法属圭亚那蜱虫有关的丝虫新属。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-15 DOI:10.1051/parasite/2024015
Marjorie Bruley, Olivier Duron
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引用次数: 0

摘要

双瓣线虫属的丝虫包括蜱传丝虫,可感染家养和野生脊椎动物宿主,但它们在许多情况下仍未得到充分研究。在这项研究中,我们对最近在南美洲法属圭亚那的两个蜱种中发现的一种类双瓣线虫丝虫(DLF)进行了分子鉴定。虽然 cox1 线粒体基因是最初描述 DLF 的唯一测序标记,但它的分类以及与其他双棘丝虫成员的系统发育关系尚不清楚。因此,我们通过对另外六个基因标记进行测序,更好地描述了 DLF 的特征,并进行了系统发育分析。根据这一多焦点分型方案,DLF 与已知的丝核藻属、种或公共数据库中的其他序列存在显著差异,这表明它有可能被归类为 Dipetalonema 系中的一个新属。系统进化分析进一步揭示了 DLF 与所有其他与蛔虫(蜱和螨)相关的丝虫之间的密切进化关系,它们在双鞭毛虫系中属于一个强大的单系亚支系。总之,这些发现证实了一个专门的、由蛔虫传播的丝虫群体的存在,并强调了对其流行病学和对动物健康的潜在影响进行全面调查的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multi-locus sequence analysis unveils a novel genus of filarial nematodes associated with ticks in French Guiana.

Filarial nematodes of the Dipetalonema lineage include tick-borne filarioids that infect both domestic and wild vertebrate hosts, but they remain understudied in many cases. In this study, we conducted a molecular characterization of a Dipetalonema-like filarioid (DLF) recently identified in two tick species in French Guiana, South America. While the cox1 mitochondrial gene was the sole marker initially sequenced for describing DLF, its classification and phylogenetic relationship with other members of the Dipetalonema lineage were unclear. Therefore, we better characterized DLF through the sequencing of six additional gene markers and conducted phylogenetic analyses. Based on this multi-locus typing scheme, DLF exhibited significant divergence from known genera and species of filarioids, or other sequences available in public databases, suggesting its potential classification as a novel genus within the Dipetalonema lineage. Phylogenetic analyses further unveiled a close evolutionary relationship between DLF and all other filarioids associated with Acari (ticks and mites) within a robust monophyletic subclade in the Dipetalonema lineage. Overall, these findings confirm the existence of a specialized, Acari-borne group of filarioids and underscore the need for comprehensive investigations into their epidemiology and potential impact on animal health.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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