一种新分离的土壤细菌利用饲料批量发酵法从甘油中高效生产 1,3-丙二醇

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Selim Ashoor, Zhuang Yao, Chan Woo Song, Haeng Lim Lee, Hyeon Jeong Seong, Sampathkumar Palaniswamy, Jong Myoung Park, Hyohak Song, Yu-Sin Jang
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引用次数: 0

摘要

从土壤、反刍动物胃液和发酵食品等不同来源共获得了 817 株类乳酸杆菌分离物。对这些分离物进行了筛选,以选出生产 1,3-丙二醇(1,3-PDO)的最佳分离物。根据 16S rRNA 基因序列,筛选出 SY235 分离物,并将其命名为reuteri 乳杆菌。然后将其用于饲料批量发酵生产 1,3-PDO。1,3-PDO 的最高滴度(52.6 ± 2.4 克/升)在 60 小时后达到,对应的产量为 0.55 ± 0.01 克/克甘油,生产率为 0.88 ± 0.04 克/升/小时。培养 60 小时后,没有观察到进一步的生产。这些结果表明,新分离的 L. reuteri SY235 菌株能有效地将甘油转化为 1,3-PDO。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Efficient production of 1,3-propanediol from glycerol by a newly isolated soil bacterium using fed-batch fermentation

Efficient production of 1,3-propanediol from glycerol by a newly isolated soil bacterium using fed-batch fermentation

A total of 817 Lactobacillus-like isolates were obtained from different sources, including soil, ruminant gastric juice, and fermented foods. They were screened to select the best isolate for the production of 1,3-propanediol (1,3-PDO). The isolate SY235 was selected and designated as Lactobacillus reuteri based on the 16S rRNA gene sequence. It was then employed in fed-batch fermentation for producing 1,3-PDO. The highest titer of 1,3-PDO (52.6 ± 2.4 g/L) was attained at 60 h, corresponding to a yield of 0.55 ± 0.01 g/g glycerol and a productivity of 0.88 ± 0.04 g/L/h. No further production was observed after 60 h of incubation. These results demonstrate that the newly isolated strain L. reuteri SY235 can efficiently transform glycerol into 1,3-PDO.

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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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