Van Dat Doan, Thi Lan Pham, Tuan Anh Nguyen, Van Cuong Bui, Xuan Minh Vu, Thi Lan Huong Nguyen, Thi My Hanh Le, Hai Khoa Le, Thanh Tung Mai, Dai lam Tran
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引用次数: 0
摘要
本研究通过原位一锅法制备了一种新型木瓜种子基活性炭/Fe3O4/AgNPs 纳米复合材料(MAC/Ag),并将其用作抗坏血酸(AA)比色检测的多功能过氧化物酶模拟物。MAC/Ag 纳米复合材料具有增强的过氧化物酶样活性,可在过氧化氢(H2O2)存在下催化 3,3,5,5-四甲基联苯胺(TMB)氧化并产生比色产物。通过优化实验条件,MAC/Ag-TMB-H2O2 检测平台显示出对 AA 的出色灵敏度,在 25-500 µM 的线性范围内,检测限低至 0.056 µM,令人印象深刻。值得注意的是,该平台具有出色的重复使用性和稳定性,即使在多次重复使用后仍能保持高水平的活性。对基本机制的全面研究表明,羟基自由基起着关键作用。木瓜汁中 AA 的成功定量分析验证了 MAC/Ag-TMB-H2O2 分析法的适用性。这些发现凸显了 MAC/Ag 纳米复合材料作为一种可靠且可重复使用的 AA 检测纳米酶的潜力,为环境监测、生物医学诊断和药物分析提供了广阔的前景。
Activated Carbon/Fe3O4/AgNPs Nanocomposites: In situ Synthesis from Papaya Seeds and Application for Ascorbic acid Detection
In this work, a novel papaya seed-based activated carbon/Fe3O4/AgNPs nanocomposite (MAC/Ag) was prepared via a one-pot in situ approach and used as a versatile peroxidase mimic for colorimetric detection of ascorbic acid (AA). The MAC/Ag nanocomposite exhibited enhanced peroxidase-like activity, catalyzing the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2) and yielding colorimetric products. By optimizing the experimental conditions, the MAC/Ag-TMB-H2O2 detection platform displayed outstanding sensitivity towards AA, with an impressively low detection limit of 0.056 µM in the linear range of 25–500 µM. Notably, the platform demonstrated remarkable reusability and stability, maintaining a high level of activity even after numerous reuse cycles. The comprehensive investigation of the underlying mechanism revealed the crucial role of hydroxyl radicals. The successful quantitative analysis of AA in papaya juice verified the applicability of the MAC/Ag-TMB-H2O2 assay. These findings highlight the potential of the MAC/Ag nanocomposite as a reliable and reusable detection nanozyme for AA, offering promising prospects in environmental monitoring, biomedical diagnostics, and pharmaceutical analysis.
期刊介绍:
The journal publishes the following types of papers: (a) original and important research;
(b) authoritative comprehensive reviews or short overviews of topics of current
interest; (c) brief but urgent communications on new significant research; and (d)
commentaries intended to foster the exchange of innovative or provocative ideas, and
to encourage dialogue, amongst researchers working in different cluster
disciplines.