高钠摄入对慢性肾病大鼠模型中肠道紧密连接结构和细菌毒素渗透性的影响

IF 4.7 3区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
María de la Luz Villela-Torres , María-del-Carmen Prado-Uribe , Marcela Ávila Díaz , Héctor Quezada Pablo , Elizabeth Soria-Castro , Nuria Esturau Escofet , Catalina Elizabeth Flores Maldonado , Ramón Paniagua
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引用次数: 0

摘要

导言尿毒症改变了肠道结构和通透性,使细菌毒素在慢性肾衰竭(CKD)期间从肠腔转移到血液中。目的 研究在 CKD 大鼠模型中,钠摄入量对源自肠道的尿毒症毒素、硫酸吲哚酯(IS)和硫酸对甲酚酯(pCS)的血浆浓度以及肠道上皮紧密连接基因和蛋白质表达的影响。方法将Sham手术大鼠(对照组,CG)和六分之五肾切除大鼠(5/6Nx)随机分配给低钠(LNa)、正常钠(NNa)或高钠(HNa)饮食、结果HNa 5/6Nx 组在 8 周和 12 周时的 IS 和 pCS 浓度高于 CG、NNa 和 LNa 组。此外,与 CG、NNa 和 LNa 相比,HNa 5/6Nx 组的 claudin-4 基因和蛋白表达量减少。与 CG 组相比,HNa 组的闭塞素基因表达量减少。与 CG、NNa 和 LNa 组相比,HNa 组的闭锁素蛋白表达更少。与 TEM 中的 NNa 和 LNa 相比,HNa 的肠道上皮紧密连接出现扩张。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of High Sodium Intake on Gut Tight Junctions’ Structure and Permeability to Bacterial Toxins in a Rat Model of Chronic Kidney Disease

Introduction

Uremic toxicity changes the gut structure and permeability, allowing bacterial toxins to translocate from the lumen to the blood during chronic kidney failure (CKD). Clinical fluid overload and tissue edema without uremia have similar effects but have not been adequately demonstrated and analyzed in CKD.

Aims

To investigate the effect of sodium intake on the plasma concentration of gut-derived uremic toxins, indoxyl sulfate (IS), and p-cresyl sulfate (pCS) and the expression of genes and proteins of epithelial gut tight junctions in a rat model of CKD.

Methods

Sham-operated (control group, CG) and five-sixths nephrectomized (5/6Nx) Sprague-Dawley rats were randomly assigned to low (LNa), normal (NNa), or high sodium (HNa) diets., Animals were then sacrificed at 8 and 12 weeks and analyzed for IS and pCS plasma concentrations, as well as for gene and protein expression of thigh junction proteins, and transmission electron microscopy (TEM) in colon fragments.

Results

The HNa 5/6Nx groups had higher concentrations of IS and pCS than CG, NNa, and LNa at eight and twelve weeks. Furthermore, HNa 5/6Nx groups had reduced expression of the claudin-4 gene and protein than CG, NNa, and LNa. HNa had reduced occludin gene expression compared to CG. Occludin protein expression was more reduced in HNa than in CG, NNa, and LNa. The gut epithelial tight junctions appear dilated in HNa compared to NNa and LNa in TEM.

Conclusion

Dietary sodium intake and fluid overload have a significant role in gut epithelial permeability in the CKD model.

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来源期刊
Archives of Medical Research
Archives of Medical Research 医学-医学:研究与实验
CiteScore
12.50
自引率
0.00%
发文量
84
审稿时长
28 days
期刊介绍: Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.
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