Chen Chao, Shiqing Huang, Cuiping Wang, Rong Sun, Jinglin Yu, Les Copeland, Shujun Wang
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引用次数: 0
摘要
由于不同类别的生物大分子(多糖、蛋白质和脂质)在化学和结构上固有的复杂性和不相容性,利用它们之间错综复杂的相互作用开发超分子组装体的新概念尚未出现。在此,我们以直链淀粉(AM)、月桂酸(LA)和β-乳球蛋白(βLG)为例,报告了多种生物大分子之间的相互作用机制及其组装体的结构和消化特性。AM、LA 和 βLG 通过 AM 和 LA 之间的范德华力以及 AM 和 βLG 之间的高亲和力相互作用,形成水溶性三元复合物。这些纳米球具有很强的抗淀粉溶解性,因此能被肠道微生物群很好地利用,包括增加短链脂肪酸含量和塑造细菌群落。这项工作说明了 AM、LA 和 βLG 的复合及其从无序到有序的组装,为非共价相互作用驱动的多种生物大分子的组装提供了潜在的理论依据,并为超分子生物材料的开发提供了巨大的潜力。
Interaction between amylose, fatty acid, and β-lactoglobulin to study multiple biomacromolecules self-assembly and application
New concept for the development of supramolecular assemblies from intricate interactions between different classes of biomacromolecules (polysaccharides, proteins and lipids) is yet to come, due to their intrinsic chemical and structural complexity and incompatibility. Herein, we report an interaction mechanism among multiple biomacromolecules, and the structural and digestive properties of their assemblies using amylose (AM), lauric acid (LA), and β-lactoglobulin (βLG) as exemplars. AM, LA, and βLG interact to form a water-soluble ternary complex through van der Waals forces between AM and LA and high affinity binding between AM and βLG, which can further assemble into uniform-sized, semi-crystalline nanospheres under certain thermodynamic conditions. These nanospheres are substantially resistant to amylolysis, thus can be well utilized by gut microbiota, including increasing short-chain fatty acid levels and shaping bacterial communities. Illustrating the complexation of AM, LA, and βLG and their assemblies from disorder to order, this work offers potential rationale of assemblies for multiple biomacromolecules driven by non-covalent interactions and substantial potentials for supramolecular biomaterials development.