{"title":"韩国一家大学医院根据感染病原体调查儿科感染性急性肠胃炎患者肠道微生物群的多样性。","authors":"","doi":"10.1016/j.pedneo.2024.01.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Acute gastroenteritis (AGE) is a common cause of pediatric morbidity and mortality worldwide. AGE can cause an imbalance in the intestinal microbiota. This study aimed to investigate the diversity of the gut microbiome in Korean children hospitalized for infectious AGE at a university hospital.</p></div><div><h3>Methods</h3><p>A total of 23 stool samples from patients aged 5 months to 11 years with AGE were analyzed. Thirteen convalescent stool samples were collected 1 month after discharge. Multiplex polymerase chain reaction (PCR) for the five viruses and 16 bacteria-specific AGE pathogens (PowerChek Multiplex Real time PCR Kit, Seoul, Korea), and 16s rRNA sequencing (Illumina MiSeq Sequencing system, Illumina, USA) were performed.</p></div><div><h3>Results</h3><p>According to the results of multiplex PCR for causative pathogens, the microbiome taxonomic profile (MTP) of the gut microbiome in three groups of AGE, norovirus AGE (n = 11), <em>Campylobacter</em> AGE (n = 7) and <em>Salmonella</em> AGE (n = 5) was compared. The phylum Actinobacteria was significantly more abundant in the norovirus AGE (<em>P</em> = 0.011), whereas the phylum Proteobacteria was significantly more abundant in <em>Salmonella</em> AGE (<em>P</em> = 0.012). Alpha diversity, which indicates species richness and diversity, showed no statistical differences. However, beta diversity, representing the similarity in MTP between norovirus, <em>Campylobacter</em>, and <em>Salmonella</em> AGE, was significantly different (<em>P</em> = 0.007). In convalescence, compared with their corresponding AGE samples, the phylum Firmicutes; and the lower taxa Christensenellaceae (<em>P</em> = 0.0152) and Lachnospiraceae (<em>P</em> = 0.0327) were significantly increased.</p></div><div><h3>Conclusions</h3><p>In pediatric AGE, the type of infectious agent can affect the diversity and dominance of gut microbiota in pediatric patients. Furthermore, healthy gut bacteria increased during the period of 1 month after infection, allowing a return to a healthy state without causing long-term dysbiosis.</p></div>","PeriodicalId":56095,"journal":{"name":"Pediatrics and Neonatology","volume":"65 5","pages":"Pages 476-481"},"PeriodicalIF":2.3000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1875957224000214/pdfft?md5=ca99bdf0b8d7397c23de6c8705e8549e&pid=1-s2.0-S1875957224000214-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Investigation of gut microbiota diversity according to infectious agent in pediatric infectious acute gastroenteritis in a Korean university hospital\",\"authors\":\"\",\"doi\":\"10.1016/j.pedneo.2024.01.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Acute gastroenteritis (AGE) is a common cause of pediatric morbidity and mortality worldwide. AGE can cause an imbalance in the intestinal microbiota. This study aimed to investigate the diversity of the gut microbiome in Korean children hospitalized for infectious AGE at a university hospital.</p></div><div><h3>Methods</h3><p>A total of 23 stool samples from patients aged 5 months to 11 years with AGE were analyzed. Thirteen convalescent stool samples were collected 1 month after discharge. Multiplex polymerase chain reaction (PCR) for the five viruses and 16 bacteria-specific AGE pathogens (PowerChek Multiplex Real time PCR Kit, Seoul, Korea), and 16s rRNA sequencing (Illumina MiSeq Sequencing system, Illumina, USA) were performed.</p></div><div><h3>Results</h3><p>According to the results of multiplex PCR for causative pathogens, the microbiome taxonomic profile (MTP) of the gut microbiome in three groups of AGE, norovirus AGE (n = 11), <em>Campylobacter</em> AGE (n = 7) and <em>Salmonella</em> AGE (n = 5) was compared. The phylum Actinobacteria was significantly more abundant in the norovirus AGE (<em>P</em> = 0.011), whereas the phylum Proteobacteria was significantly more abundant in <em>Salmonella</em> AGE (<em>P</em> = 0.012). Alpha diversity, which indicates species richness and diversity, showed no statistical differences. However, beta diversity, representing the similarity in MTP between norovirus, <em>Campylobacter</em>, and <em>Salmonella</em> AGE, was significantly different (<em>P</em> = 0.007). In convalescence, compared with their corresponding AGE samples, the phylum Firmicutes; and the lower taxa Christensenellaceae (<em>P</em> = 0.0152) and Lachnospiraceae (<em>P</em> = 0.0327) were significantly increased.</p></div><div><h3>Conclusions</h3><p>In pediatric AGE, the type of infectious agent can affect the diversity and dominance of gut microbiota in pediatric patients. Furthermore, healthy gut bacteria increased during the period of 1 month after infection, allowing a return to a healthy state without causing long-term dysbiosis.</p></div>\",\"PeriodicalId\":56095,\"journal\":{\"name\":\"Pediatrics and Neonatology\",\"volume\":\"65 5\",\"pages\":\"Pages 476-481\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1875957224000214/pdfft?md5=ca99bdf0b8d7397c23de6c8705e8549e&pid=1-s2.0-S1875957224000214-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pediatrics and Neonatology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875957224000214\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PEDIATRICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pediatrics and Neonatology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875957224000214","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PEDIATRICS","Score":null,"Total":0}
引用次数: 0
摘要
背景:急性肠胃炎(AGE)是全球儿童发病和死亡的常见原因。AGE 可导致肠道微生物群失衡。本研究旨在调查在一家大学医院因感染性 AGE 而住院的韩国儿童肠道微生物组的多样性:分析了 23 份粪便样本,这些样本来自 5 个月至 11 岁的 AGE 患者。出院 1 个月后收集了 13 份康复粪便样本。对 5 种病毒和 16 种细菌特异性 AGE 病原体(PowerChek Multiplex Real time PCR Kit,韩国首尔)进行多重聚合酶链反应(PCR),并对 16s rRNA 进行测序(Illumina MiSeq 测序系统,美国 Illumina 公司):结果:根据致病菌的多重 PCR 结果,比较了三组 AGE(诺如病毒 AGE(n = 11)、弯曲杆菌 AGE(n = 7)和沙门氏菌 AGE(n = 5))的肠道微生物组分类概况(MTP)。诺如病毒 AGE 中放线菌门的数量明显较多(P = 0.011),而沙门氏菌 AGE 中变形菌门的数量明显较多(P = 0.012)。表示物种丰富度和多样性的α多样性没有显示出统计学差异。然而,代表诺如病毒、弯曲杆菌和 AGE 沙门氏菌之间 MTP 相似性的贝塔多样性却有显著差异(P = 0.007)。与相应的 AGE 样本相比,康复期样本中的真菌门、低等类群克里斯滕森菌科(P = 0.0152)和拉赫诺斯皮拉菌科(P = 0.0327)明显增加:结论:在小儿 AGE 中,感染病原体的类型会影响小儿患者肠道微生物群的多样性和优势。此外,健康的肠道细菌在感染后的 1 个月内有所增加,可恢复到健康状态,而不会导致长期的菌群失调。
Investigation of gut microbiota diversity according to infectious agent in pediatric infectious acute gastroenteritis in a Korean university hospital
Background
Acute gastroenteritis (AGE) is a common cause of pediatric morbidity and mortality worldwide. AGE can cause an imbalance in the intestinal microbiota. This study aimed to investigate the diversity of the gut microbiome in Korean children hospitalized for infectious AGE at a university hospital.
Methods
A total of 23 stool samples from patients aged 5 months to 11 years with AGE were analyzed. Thirteen convalescent stool samples were collected 1 month after discharge. Multiplex polymerase chain reaction (PCR) for the five viruses and 16 bacteria-specific AGE pathogens (PowerChek Multiplex Real time PCR Kit, Seoul, Korea), and 16s rRNA sequencing (Illumina MiSeq Sequencing system, Illumina, USA) were performed.
Results
According to the results of multiplex PCR for causative pathogens, the microbiome taxonomic profile (MTP) of the gut microbiome in three groups of AGE, norovirus AGE (n = 11), Campylobacter AGE (n = 7) and Salmonella AGE (n = 5) was compared. The phylum Actinobacteria was significantly more abundant in the norovirus AGE (P = 0.011), whereas the phylum Proteobacteria was significantly more abundant in Salmonella AGE (P = 0.012). Alpha diversity, which indicates species richness and diversity, showed no statistical differences. However, beta diversity, representing the similarity in MTP between norovirus, Campylobacter, and Salmonella AGE, was significantly different (P = 0.007). In convalescence, compared with their corresponding AGE samples, the phylum Firmicutes; and the lower taxa Christensenellaceae (P = 0.0152) and Lachnospiraceae (P = 0.0327) were significantly increased.
Conclusions
In pediatric AGE, the type of infectious agent can affect the diversity and dominance of gut microbiota in pediatric patients. Furthermore, healthy gut bacteria increased during the period of 1 month after infection, allowing a return to a healthy state without causing long-term dysbiosis.
期刊介绍:
Pediatrics and Neonatology is the official peer-reviewed publication of the Taiwan Pediatric Association and The Society of Neonatology ROC, and is indexed in EMBASE and SCOPUS. Articles on clinical and laboratory research in pediatrics and related fields are eligible for consideration.