从墨西哥一家医院的医源性感染患者中分离出的对碳青霉烯类和阿曲南易感和耐药铜绿假单胞菌菌株的基因组比较分析。

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
María José Martínez-Gallardo, Claudia Villicaña, Martha Yocupicio-Monroy, Sofía Lizeth Alcaraz-Estrada, Juana Salazar-Salinas, Omar Fernando Mendoza-Vázquez, Gabriel Damazo-Hernández, Josefina León-Félix
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引用次数: 0

摘要

铜绿假单胞菌(Pseudomonas aeruginosa,PA)是一种重要的机会性病原体,会对免疫力低下的患者造成不同程度的感染。在铜绿假单胞菌的附属基因组中,不同菌株在毒力、抗生素耐药性和生物膜形成方面存在差异,导致了耐多药菌株的出现。对从墨西哥一家医院的医源性感染患者身上分离出的 17 株菌株的基因组序列进行了基因组和系统发育分析,并检测了抗生素耐药性基因、毒力基因和生物膜形成基因。在 17 株菌株中,有 15 株至少对两种碳青霉烯类药物美罗培南、亚胺培南和单内酰胺类药物阿兹卓南具有耐药性。所有菌株都检测到了抗生素耐药基因(mexA、mexB 和 oprM)和生物膜形成基因(pslA 和 pslD)。不同菌株的附属基因组大小存在差异。菌株具有不同的序列类型,其中 7 株菌株的序列类型与全球高风险流行性 PA 克隆有关。在全球 PA 菌株中,所有菌株都分为两组。在 17 株菌株中,主要发现了对氨基糖苷类和β-内酰胺类的水平获得抗性基因,以及 230 至 240 个编码毒力因子的基因。所研究的菌株在附属基因组、抗生素耐药性和毒力基因方面存在差异。通过这些特征,我们提供了临床相关 PA 菌株基因组多样性的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comparative genomic analysis of Pseudomonas aeruginosa strains susceptible and resistant to carbapenems and aztreonam isolated from patients with healthcare-associated infections in a Mexican hospital.

Comparative genomic analysis of Pseudomonas aeruginosa strains susceptible and resistant to carbapenems and aztreonam isolated from patients with healthcare-associated infections in a Mexican hospital.

Pseudomonas aeruginosa (PA) is an important opportunistic pathogen that causes different infections on immunocompromised patients. Within PA accessory genome, differences in virulence, antibiotic resistance and biofilm formation have been described between strains, leading to the emergence of multidrug-resistant strains. The genome sequences of 17 strains isolated from patients with healthcare-associated infections in a Mexican hospital were genomically and phylogenetically analyzed and antibiotic resistance genes, virulence genes, and biofilm formation genes were detected. Fifteen of the 17 strains were resistant to at least two of the carbapenems meropenem, imipenem, and the monobactam aztreonam. The antibiotic resistance (mexA, mexB, and oprM) and the biofilm formation (pslA and pslD) genes were detected in all strains. Differences were found between strains in accessory genome size. The strains had different sequence types, and seven strains had sequence types associated with global high risk epidemic PA clones. All strains were represented in two groups among PA global strains. In the 17 strains, horizontally acquired resistance genes to aminoglycosides and beta-lactams were found, mainly, and between 230 and 240 genes that encode virulence factors. The strains under study were variable in terms of their accessory genome, antibiotic resistance, and virulence genes. With these characteristics, we provide information about the genomic diversity of clinically relevant PA strains.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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