生物仿生涂层和水溶性蛋壳膜蛋白交联钛合金螺钉的生物相容性和骨结合。

Q2 Biochemistry, Genetics and Molecular Biology
Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-10-24 DOI:10.61186/ibj.3939
Mehmet Bahadır İlik, Ercan Durmuş, İlhami Çelik
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引用次数: 0

摘要

背景:牙科和整形外科植入物的表面特性与其骨结合率直接相关。对种植体表面进行涂层和/或改性可能会缩短愈合时间。在本研究中,我们旨在研究由刷石表面涂层和交联水溶性蛋壳膜蛋白组成的混合表面在体内条件下对钛螺钉骨结合的影响:在新西兰兔胫骨前内侧区域单皮质植入 20 颗钛合金螺钉。其中 10 根螺钉未经处理,用作对照组。其余 10 根螺钉涂有磷酸钙,然后与鸵鸟蛋壳膜蛋白交联。所有兔子均在手术后六周处死。用 µ-CT、组织学和组织形态计量学方法对螺钉周围组织进行了评估:μ-CT评估表明,实验组的平均BSA和TbN明显高于对照组(P ˂ 0.05)。对照组和实验组的 BV、TbSp、TbTh 和 BMD 评分相当接近(P > 0.05)。实验组的血管化评分明显高于对照组(4.29% 对 0.92%)。没有观察到移植物抗宿主反应的迹象:我们的研究结果表明,用鸵鸟蛋壳膜蛋白交联的磷酸钙涂覆钛合金种植体可增加种植部位的骨表面积、骨小梁数量和血管化程度,从而提高钛合金螺钉的骨结合率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biocompatibility and Osseointegration of the Biomimetically Coated and Water-Soluble Eggshell Membrane Protein Cross-Linked Ti Alloy Screws.

Background: The surface properties of dental and orthopedic implants are directly related to their osseointegration rate. Coating and/or modifying the implant surface might reduce the time of healing. In this study, we aimed to examine the effects of a hybrid surface consisting of a brushite surface coating and cross-linked water-soluble eggshell membrane protein on the osseointegration of titanium (Ti) screws under in vivo conditions.

Methods: Twenty Ti alloy screws were implanted monocortically in anteromedial regions of New Zealand rabbit tibiae. Ten screws were untreated and used as controls. The remaining 10 screws were coated with calcium phosphate and following cross-linked with ostrich eggshell membrane protein. All rabbits were sacrificed six weeks after the surgery. Peri-screw tissues were evaluated by micro-computed tomography (µ-CT), histological and histomorphometrical methods.

Results: The μ-CT assessments indicated that the experimental group had significantly higher mean bone surface area (BSA) and trabeculae number (TbN) than those of the control group (p ˂ 0.05). Bone surface area (BV), trabecular separation (TbSp), trabecular thickness (TbTh), and bone mineral density (BMD) scores of the control and experimental groups were quite similar (p > 0.05). The vascularization score of the experimental group was significantly higher than the control group (4.29 vs. 0.92%). No sign of the graft-versus-host reaction was observed.

Conclusion: Our findings reveal that coating Ti alloy implants with calcium phosphate cross-linked with ostrich eggshell membrane protein increases the osseointegration of Ti alloy screws by increasing the bone surface area, number of trabeculae and vascularization in the implant site.

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来源期刊
Iranian Biomedical Journal
Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.20
自引率
0.00%
发文量
42
审稿时长
8 weeks
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