基于 ITS、ISSR 和 SRAP 标记的鸡冠花遗传多样性和遗传关系分析

IF 1.4 4区生物学 Q4 MYCOLOGY

International journal of medicinal mushrooms Pub Date : 2024-03-01 DOI:10.1615/intjmedmushrooms.2024053182

Ping Du, Hongyan He, Niya Wu, Tianxu Cao, BaoKai Cui

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引用次数: 0

摘要

我国角杯藻（A. polytricha）野生资源丰富，角杯藻的遗传多样性和遗传关系分析可为种质资源利用和创新以及分子标记辅助育种提供依据。本研究基于ITS序列分析，将采集到的22株鸡冠花鉴定为鸡冠花，并利用ISSR和SRAP标记检测了鸡冠花的遗传多样性。结果表明，11个选定的ISSR引物共扩增出415条带，平均每个引物扩增出37.73条带，Ne、I和H的平均值分别为1.302、0.368和0.219。10 个 SRAP 引物共扩增了 450 条带，平均每个引物扩增 45 条带，Ne、I 和 H 的平均值分别为 1.263、0.302 和 0.183。基于 ISSR-SRAP 标记数据的非加权对群法与算术平均数分析表明，被测菌株间的遗传相似系数为 0.73-0.97，菌株间的遗传相似系数为 0.742，可分为 5 组，与区域分布有一定的相关性。基于 ISSR-SRAP 数据的 PCOA 和种群结构分析也得出了类似的结果。这些结果表明了野生鸡冠花的遗传多样性和差异性，为鸡冠花资源的分类、育种、种质创新、利用和品种保护提供了理论参考。

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Genetic diversity and genetic relationship analysis of Auricularia cornea Based on ITS, ISSR and SRAP Markers

Wild resources of Auricularia cornea (= A. polytricha) are abundant in China, and genetic diversity and genetic relationships analysis of A. cornea can provide basis for germplasm resource utilization and innovation and molecular marker-assisted breeding. In this study, 22 Auricularia strains collected were identified as A. cornea based on ITS sequence analysis, and its genetic diversity was examined by ISSR and SRAP markers. The results showed that a total of 415 bands were amplified by 11 selected ISSR primers, with an average amplification of 37.73 bands per primer, and the mean values of Ne, I and H were 1.302, 0.368 and 0.219, respectively. A total of 450 bands were amplified by 10 SRAP primers, with an average of 45 bands per primer, and the average of Ne, I and H were 1.263, 0.302 and 0.183, respectively. The unweighted pair-group method with arithmetic means analysis based on ISSR-SRAP marker data revealed that the genetic similarity coefficient between the tested strains was 0.73-0.97, and the strains could be divided into five groups at 0.742, which had a certain correlation with regional distribution. The results of PCOA and population structure analysis based on ISSR-SRAP data also produced similar results. These results demonstrate the genetic diversity and distinctness among wild A. cornea and provide a theoretical reference for the classification, breeding, germplasm innovation, utilization, and variety protection of A. cornea resources.

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来源期刊

International journal of medicinal mushrooms 医学-药学

CiteScore

2.60

自引率

16.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.