活细胞亚细胞动态的快速容积多焦结构照明显微技术

IF 2.9 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2024-03-11 DOI:10.1364/boe.516261

Maximilian Lukas Senftleben, Antone Bajor, Eduardo Hirata, Sara Abrahamsson, and Hjalmar Brismar

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引用次数: 0

摘要

利用二维结构照明显微镜（SIM）可以研究亚细胞结构的纳米级动态。与典型的宽场相比，该方法可提高采集分辨率。对于三维样品，由于需要连续采集二维平面来创建体积，因此采集速度受到固有限制。在此，我们介绍了多焦点 SIM 的开发情况，其设计目的是通过使用快速同步的电子光学元件来提供高容积帧频。我们通过记录活细胞中微管和内质网的动态，以每秒高达 2.3 个超分辨率体积（总体积为 30 × 30 × 1.8 µm3）的速度，展示了该显微镜的高体积成像能力。

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Fast volumetric multifocus structured illumination microscopy of subcellular dynamics in living cells

Studying the nanoscale dynamics of subcellular structures is possible with 2D structured illumination microscopy (SIM). The method allows for acquisition with improved resolution over typical widefield. For 3D samples, the acquisition speed is inherently limited by the need to acquire sequential two-dimensional planes to create a volume. Here, we present a development of multifocus SIM designed to provide high volumetric frame rate by using fast synchronized electro-optical components. We demonstrate the high volumetric imaging capacity of the microscope by recording the dynamics of microtubule and endoplasmatic reticulum in living cells at up to 2.3 super resolution volumes per second for a total volume of 30 × 30 × 1.8 µm³.

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.