用于生成浓度梯度和溶液组合的高通量 3D 微流体芯片

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2024-03-12 DOI:10.1039/D3LC00822C
Mingwei Zhao, Jing Yang, Zhenqing Li, Yuan Zeng, Chunxian Tao, Bo Dai, Dawei Zhang and Yoshinori Yamaguchi
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引用次数: 0

摘要

浓度梯度的形成和多种溶液的混合组合在生物医学研究领域具有重要价值。然而,现有的单药或双药溶液浓度梯度发生器无法同时实现多种浓度梯度形成和混合溶液组合。此外,整个系统体积庞大,需要昂贵的辅助设备,可能导致复杂的操作。为解决这一问题,我们提出了一种三维微流控芯片。它由溶液加载寄存器、浓度梯度和混合发生器以及溶液收集器组成。它可以实现一至三种溶液的混合,并提供各种装载位置组合,以促进多种类型的浓度梯度和混合组合。我们采用了比色法和荧光法来测试微流控芯片的效率。结果显示,十种浓度从 31.9% 到 98.3% 不等的溶液被均匀生成,并获得了二十种混合溶液组合。我们预计,三维微流控芯片将成为多溶液梯度稀释和组合的理想选择,如各种药物筛选应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

High-throughput 3D microfluidic chip for generation of concentration gradients and mixture combinations†

High-throughput 3D microfluidic chip for generation of concentration gradients and mixture combinations†

Concentration gradient generation and mixed combinations of multiple solutions are of great value in the field of biomedical research. However, existing concentration gradient generators for single or two-drug solutions cannot simultaneously achieve multiple concentration gradient formations and mixed solution combinations. Furthermore, the whole system was huge, and required expensive auxiliary equipment, which may lead to complex operations. To address this problem, we devised a novel 3D microchannel network design, which is capable of creating all the desired mixture combinations and concentration gradients of given small amounts of the input solutions. As a proof of concept, the device we presented was verified by both colorimetric and fluorescence detection methods to test the efficiency. This can enable the implementation of one to three solutions with no driving pump and facilitate unique multiple types of more concentration gradients and mixture combinations in a single operation. We envision that this will be a promising candidate for the development of simplified methods for screening of the appropriate concentration and combination, such as various drug screening applications.

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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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