Cynthia Oluchi Onwusereaka, Juliana Jalaludin, Khairul Nizam Mohd Isa, Syafinaz Binti Amin Nordin, Suhaili Abubakar, Veronica Chua Poo Choo
{"title":"暴露于空气污染物的学龄前儿童微生物组的靶向元基因组学鉴定及其与呼吸道健康症状、过敏和湿疹的关系","authors":"Cynthia Oluchi Onwusereaka, Juliana Jalaludin, Khairul Nizam Mohd Isa, Syafinaz Binti Amin Nordin, Suhaili Abubakar, Veronica Chua Poo Choo","doi":"10.1007/s11869-024-01545-y","DOIUrl":null,"url":null,"abstract":"<div><p>Microorganisms are an important aspect of the environment which have significant effects on human health. Children are more vulnerable to the effects of microbial pollutants, pathogens and allergens. Microbial components of indoor air cannot be overemphasized due to its vastness and ubiquitous nature. This is a cross sectional comparative study which analysed microbiome in 10 preschools according to their level of exposure to outdoor air pollutant sources using amplicon sequencing technique. A total of 20 dust samples were collected from indoor and outdoor environment and analysed for the presence of bacteria and fungi. A detailed questionnaire was used to assess the respiratory health status of the respondents. Indoor air quality was assessed by measuring the level of particulate matter (PM<sub>2.5</sub> and PM<sub>10</sub>), temperature, relative humidity, air velocity, and CO<sub>2</sub>. Exposed area recorded higher level of PM<sub>10</sub> and CO<sub>2</sub> (<i>p</i> <0.05) while temperature, relative humidity and air velocity were higher in the comparative area (<i>p</i> <0.05). Health symptoms were significantly higher in exposed area for cough, eczema and allergy symptoms (<i>p</i> <0.05). Predominant bacteria species includes <i>Acinetobacter_radioresistens, Acinetobacter_seifertii</i>, <i>Lactobaccillus_iners, Pseudomonas_B_luteola,</i> and <i>Franconibacter_pulveris</i> while predominant fungi species includes <i>Rhodosporidioboluc_fluvialis, Rhodotorula_kratochvilovae, Aspergillus_penicilloides</i>, <i>Aspergillus_gracilis, Curvularia_sorghina, Eupenidiella_venezuelensis</i>, <i>Rhodosporidiobolus_ruineniae</i>, <i>Bipolaris_papendorfii</i> and <i>Curvularia_lunata</i>. Cough symptom was strongly associated with PM<sub>10</sub> (AOR =1.27), carpeting (AOR =1.62), <i>Cladosporium dominicanum</i> (AOR =2.12) and <i>Aureobasidium gracilis</i> (AOR =2.04). Eczema was strongly associated with keeping furry pet (AOR =5.46) and <i>Rhodotorula_toruloides</i> (1.02). Allergy was strongly associated with PM<sub>10</sub> (AOR =3.23), <i>Megasporoporia_bannaensis</i> (AOR =1.43) and <i>Malassezia_restricta</i> (AOR =2.10). Preschools located close to outdoor air pollutants were more exposed to pollutants and microbiome from indoor and outdoor sources. Health symptoms were significantly associated with air pollutants, therefore proper measures should be implemented to mitigate the exposure to microbiome, infiltration of outdoor air pollutants and health effects on children.</p></div>","PeriodicalId":49109,"journal":{"name":"Air Quality Atmosphere and Health","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Targeted Metagenomics Identification of Microbiome in Preschools exposed to air Pollutants and Their Association with Respiratory Health symptom, Allergy and Eczema\",\"authors\":\"Cynthia Oluchi Onwusereaka, Juliana Jalaludin, Khairul Nizam Mohd Isa, Syafinaz Binti Amin Nordin, Suhaili Abubakar, Veronica Chua Poo Choo\",\"doi\":\"10.1007/s11869-024-01545-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microorganisms are an important aspect of the environment which have significant effects on human health. Children are more vulnerable to the effects of microbial pollutants, pathogens and allergens. Microbial components of indoor air cannot be overemphasized due to its vastness and ubiquitous nature. This is a cross sectional comparative study which analysed microbiome in 10 preschools according to their level of exposure to outdoor air pollutant sources using amplicon sequencing technique. A total of 20 dust samples were collected from indoor and outdoor environment and analysed for the presence of bacteria and fungi. A detailed questionnaire was used to assess the respiratory health status of the respondents. Indoor air quality was assessed by measuring the level of particulate matter (PM<sub>2.5</sub> and PM<sub>10</sub>), temperature, relative humidity, air velocity, and CO<sub>2</sub>. Exposed area recorded higher level of PM<sub>10</sub> and CO<sub>2</sub> (<i>p</i> <0.05) while temperature, relative humidity and air velocity were higher in the comparative area (<i>p</i> <0.05). Health symptoms were significantly higher in exposed area for cough, eczema and allergy symptoms (<i>p</i> <0.05). Predominant bacteria species includes <i>Acinetobacter_radioresistens, Acinetobacter_seifertii</i>, <i>Lactobaccillus_iners, Pseudomonas_B_luteola,</i> and <i>Franconibacter_pulveris</i> while predominant fungi species includes <i>Rhodosporidioboluc_fluvialis, Rhodotorula_kratochvilovae, Aspergillus_penicilloides</i>, <i>Aspergillus_gracilis, Curvularia_sorghina, Eupenidiella_venezuelensis</i>, <i>Rhodosporidiobolus_ruineniae</i>, <i>Bipolaris_papendorfii</i> and <i>Curvularia_lunata</i>. Cough symptom was strongly associated with PM<sub>10</sub> (AOR =1.27), carpeting (AOR =1.62), <i>Cladosporium dominicanum</i> (AOR =2.12) and <i>Aureobasidium gracilis</i> (AOR =2.04). Eczema was strongly associated with keeping furry pet (AOR =5.46) and <i>Rhodotorula_toruloides</i> (1.02). Allergy was strongly associated with PM<sub>10</sub> (AOR =3.23), <i>Megasporoporia_bannaensis</i> (AOR =1.43) and <i>Malassezia_restricta</i> (AOR =2.10). Preschools located close to outdoor air pollutants were more exposed to pollutants and microbiome from indoor and outdoor sources. Health symptoms were significantly associated with air pollutants, therefore proper measures should be implemented to mitigate the exposure to microbiome, infiltration of outdoor air pollutants and health effects on children.</p></div>\",\"PeriodicalId\":49109,\"journal\":{\"name\":\"Air Quality Atmosphere and Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-03-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Air Quality Atmosphere and Health\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11869-024-01545-y\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Air Quality Atmosphere and Health","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1007/s11869-024-01545-y","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Targeted Metagenomics Identification of Microbiome in Preschools exposed to air Pollutants and Their Association with Respiratory Health symptom, Allergy and Eczema
Microorganisms are an important aspect of the environment which have significant effects on human health. Children are more vulnerable to the effects of microbial pollutants, pathogens and allergens. Microbial components of indoor air cannot be overemphasized due to its vastness and ubiquitous nature. This is a cross sectional comparative study which analysed microbiome in 10 preschools according to their level of exposure to outdoor air pollutant sources using amplicon sequencing technique. A total of 20 dust samples were collected from indoor and outdoor environment and analysed for the presence of bacteria and fungi. A detailed questionnaire was used to assess the respiratory health status of the respondents. Indoor air quality was assessed by measuring the level of particulate matter (PM2.5 and PM10), temperature, relative humidity, air velocity, and CO2. Exposed area recorded higher level of PM10 and CO2 (p <0.05) while temperature, relative humidity and air velocity were higher in the comparative area (p <0.05). Health symptoms were significantly higher in exposed area for cough, eczema and allergy symptoms (p <0.05). Predominant bacteria species includes Acinetobacter_radioresistens, Acinetobacter_seifertii, Lactobaccillus_iners, Pseudomonas_B_luteola, and Franconibacter_pulveris while predominant fungi species includes Rhodosporidioboluc_fluvialis, Rhodotorula_kratochvilovae, Aspergillus_penicilloides, Aspergillus_gracilis, Curvularia_sorghina, Eupenidiella_venezuelensis, Rhodosporidiobolus_ruineniae, Bipolaris_papendorfii and Curvularia_lunata. Cough symptom was strongly associated with PM10 (AOR =1.27), carpeting (AOR =1.62), Cladosporium dominicanum (AOR =2.12) and Aureobasidium gracilis (AOR =2.04). Eczema was strongly associated with keeping furry pet (AOR =5.46) and Rhodotorula_toruloides (1.02). Allergy was strongly associated with PM10 (AOR =3.23), Megasporoporia_bannaensis (AOR =1.43) and Malassezia_restricta (AOR =2.10). Preschools located close to outdoor air pollutants were more exposed to pollutants and microbiome from indoor and outdoor sources. Health symptoms were significantly associated with air pollutants, therefore proper measures should be implemented to mitigate the exposure to microbiome, infiltration of outdoor air pollutants and health effects on children.
期刊介绍:
Air Quality, Atmosphere, and Health is a multidisciplinary journal which, by its very name, illustrates the broad range of work it publishes and which focuses on atmospheric consequences of human activities and their implications for human and ecological health.
It offers research papers, critical literature reviews and commentaries, as well as special issues devoted to topical subjects or themes.
International in scope, the journal presents papers that inform and stimulate a global readership, as the topic addressed are global in their import. Consequently, we do not encourage submission of papers involving local data that relate to local problems. Unless they demonstrate wide applicability, these are better submitted to national or regional journals.
Air Quality, Atmosphere & Health addresses such topics as acid precipitation; airborne particulate matter; air quality monitoring and management; exposure assessment; risk assessment; indoor air quality; atmospheric chemistry; atmospheric modeling and prediction; air pollution climatology; climate change and air quality; air pollution measurement; atmospheric impact assessment; forest-fire emissions; atmospheric science; greenhouse gases; health and ecological effects; clean air technology; regional and global change and satellite measurements.
This journal benefits a diverse audience of researchers, public health officials and policy makers addressing problems that call for solutions based in evidence from atmospheric and exposure assessment scientists, epidemiologists, and risk assessors. Publication in the journal affords the opportunity to reach beyond defined disciplinary niches to this broader readership.