对聚碳酸酯-聚氨酯氧化锆复合材料用于病灶膝关节置换植入物的骨结合能力进行体外和体内评估。

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Pieter P. W. van Hugten, Ralph M. Jeuken, Erkan E. Asik, Henk Oevering, Tim J. M. Welting, Corrinus C. van Donkelaar, Jens C. Thies, Peter J. Emans, Alex K. Roth
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引用次数: 0

摘要

目前可用的病灶膝关节置换植入物(FKRI)全部或部分由金属构成,这些金属与骨和软骨组织的弹性模量相差很大。虽然钛以其出色的骨结合性著称,但在 FKRI 中的应用可能会导致潜在的应力屏蔽,而且金属植入物可能会因产生的高接触应力而导致对侧关节软骨退化。为了克服使用金属基 FKRIs 的弊端,我们开发了一种生物仿生和核磁共振兼容的双层非吸收热塑性聚碳酸酯-聚氨酯(PCU)基 FKRI。这项临床前研究的目的是评估新型 Bionate® 75D - 氧化锆(B75D-ZrO2)复合材料在体外的机械性能、生物相容性和骨传导性,以及 B75D-ZrO2 复合材料干 PCU 植入体在山羊动物模型中的骨结合情况。B75D-ZrO2 复合材料的拉伸强度和弹性模量是通过环境和生理条件下的体外机械测试确定的。通过将人间质干细胞暴露于 B75D-ZrO2 复合材料并在成骨条件下培养细胞,对体外生物相容性和骨传导性进行了评估。评估了细胞活性和矿化度,并与 Bionate® 75D (B75D) 和钛盘进行了比较。在大型动物模型中,将含有 B75D-ZrO2 骨干的植入物与含有 B75D 骨干和钛骨干的植入物进行体内骨结合比较。随访 6 个月后,进行了骨组织形态测量,以评估骨与种植体的接触面积 (BIC)。机械测试表明,B75D-ZrO2 复合材料的弹性模量与所报道的骨小梁弹性模量范围相当。B75D-ZrO2 复合材料促进细胞介导矿化的程度与钛相当。与 B75D 种植体相比,B75D-ZrO2 种植体的骨与种植体接触(BIC)得分明显更高。B75D-ZrO2 植入体的骨-植入体接触分数与钛植入体相比没有明显差异。通过将 B75D 与氧化锆复合,开发出了一种生物相容性 B75D-ZrO2 复合材料,其弹性模量接近骨小梁。体内评估显示,与 B75D 聚合物干 PCU 植入体相比,B75D-ZrO2 复合干植入体的骨结合率显著提高。B75D-ZrO2 复合材料干 PCU 植入体的骨结合率与类似的钛干金属植入体相比没有明显差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In vitro and in vivo evaluation of the osseointegration capacity of a polycarbonate-urethane zirconium-oxide composite material for application in a focal knee resurfacing implant

In vitro and in vivo evaluation of the osseointegration capacity of a polycarbonate-urethane zirconium-oxide composite material for application in a focal knee resurfacing implant

Currently available focal knee resurfacing implants (FKRIs) are fully or partially composed of metals, which show a large disparity in elastic modulus relative to bone and cartilage tissue. Although titanium is known for its excellent osseointegration, the application in FKRIs can lead to potential stress-shielding and metal implants can cause degeneration of the opposing articulating cartilage due to the high resulting contact stresses. Furthermore, metal implants do not allow for follow-up using magnetic resonance imaging (MRI).To overcome the drawbacks of using metal based FKRIs, a biomimetic and MRI compatible bi-layered non-resorbable thermoplastic polycarbonate-urethane (PCU)-based FKRI was developed. The objective of this preclinical study was to evaluate the mechanical properties, biocompatibility and osteoconduction of a novel Bionate® 75D - zirconium oxide (B75D-ZrO2) composite material in vitro and the osseointegration of a B75D-ZrO2 composite stem PCU implant in a caprine animal model. The tensile strength and elastic modulus of the B75D-ZrO2 composite were characterized through in vitro mechanical tests under ambient and physiological conditions. In vitro biocompatibility and osteoconductivity were evaluated by exposing human mesenchymal stem cells to the B75D-ZrO2 composite and culturing the cells under osteogenic conditions. Cell activity and mineralization were assessed and compared to Bionate® 75D (B75D) and titanium disks. The in vivo osseointegration of implants containing a B75D-ZrO2 stem was compared to implants with a B75D stem and titanium stem in a caprine large animal model. After a follow-up of 6 months, bone histomorphometry was performed to assess the bone-to-implant contact area (BIC). Mechanical testing showed that the B75D-ZrO2 composite material possesses an elastic modulus in the range of the elastic modulus reported for trabecular bone. The B75D-ZrO2 composite material facilitated cell mediated mineralization to a comparable extent as titanium. A significantly higher bone-to-implant contact (BIC) score was observed in the B75D-ZrO2 implants compared to the B75D implants. The BIC of B75D-ZrO2 implants was not significantly different compared to titanium implants. A biocompatible B75D-ZrO2 composite approximating the elastic modulus of trabecular bone was developed by compounding B75D with zirconium oxide. In vivo evaluation showed an significant increase of osseointegration for B75D-ZrO2 composite stem implants compared to B75D polymer stem PCU implants. The osseointegration of B75D-ZrO2 composite stem PCU implants was not significantly different in comparison to analogous titanium stem metal implants.

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来源期刊
Journal of biomedical materials research. Part A
Journal of biomedical materials research. Part A 工程技术-材料科学:生物材料
CiteScore
10.40
自引率
2.00%
发文量
135
审稿时长
3.6 months
期刊介绍: The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.
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